Auditory Thalamocortical Synaptic Transmission In Vitro

Cruikshank, Rose, Metherate · Journal of Neurophysiology · 2002 · neuroscience · 10.1152/jn.00549.2001 · record Cruikshank2002-auditory-thalamocortical-synaptic-transmissi
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Where this record's text came from. faceted 2026-08 by reading the locally held text (pdftext); every evidence string verified as an exact substring of that text before merging. Claim type is 'method' because the headline contribution is a preparation (a way of doing X), even though the paper also reports substantive mechanism findings - the Discussion lists 'Quotation withheld' A mechanism-typed sibling record could legitimately be cut from that sentence. method is tagged 'electrophysiology' rather than 'system-or-tool-building' because the characterisation work is intracellular/extracellular recording plus CSD; the tool-building aspect is captured by claim type instead. This is pdftext output in which many symbols were lost to '(cid:N)' codes (e.g. micrometre, plus/minus, less-than); I chose evidence sentences free of those artefacts so the quotes stay legible, which is why the methods quote is the chamber sentence rather than the abstract's fuller technique list. Replication status 'untested': studies using the same preparation are cited (Hsieh et al. 2000; Rose and Metherate 2001) but as parallel work, not as replication. 2026-08-14 schema-0.3 pass: every quote verified as an exact substring of the staged text and anchored at merge time; concept terms verified present; every edge endpoint an existing node.
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Tags on this paper

Every tag the record carries, each linking to every other paper in the corpus that carries it. A tag appears here whether or not its words occur in the article — the spans below are only the ones a sentence in this paper licenses. Of the 7 concept tags on this paper, all 7 are grounded in a quoted sentence.
columnar stimulation thalamocortical inputs MG stimuli on-beam stimuli IPSPs excitatory thalamic projections cortical inhibitory interneurons Thalamic glutamatergic axons excite cortical inhibitory interneurons, which then synapse on the recorded cell, producing the disynaptic fast IPSP Antidromic activation of layer 5 corticotectal cells whose axons cross the shell stimulation site, feeding back through their intracortical collaterals to generate the layer 1 sink on-beam stimulation early response late responses primary slice shell slice stimulus intensity current source density amplitudes of the early responses t-test paired t-test unpaired t-test mean Mean values ... (±SE) electrophysiology

Legend

relation to other workclaim_relationshow this paper stands to another: agrees, contradicts, extends, qualifies
claim typeclaim_typethe shape of the claim: existence, mechanism, method, critique, prediction, null
concept edgeconcept_edgesan asserted relation between two concepts, with the sentence asserting it
conceptconceptsan idea the paper works with, quoted where the paper uses it
content tagcontent_tagsa finding, definition, quantity, statistic or variable named in the paper
controlcontrolsan experiment run to eliminate a rival explanation
limitationlimitationswhat the paper says its evidence cannot support
mechanismmechanismshow the reported effect is said to come about
methodmethodhow the paper produced its evidence
phenomenonphenomenonthe effect or regularity under study
sensesensesa distinct use of a term, quoted where the paper fixes it

The paper

The full text is not reproduced here. This article is not cleared for redistribution, so the page carries the tag layer only. Its evidence sentences are withheld; the grounding status remains with each tag, and the article itself is at https://doi.org/10.1152/jn.00549.2001. The reading view exists for the papers whose licence permits it.

Every tag on this page

claim_relations 3 · claim_type 1 · concept_edges 2 · concepts 7 · content_tags 23 · controls 2 · limitations 10 · mechanisms 2 · method 1 · phenomenon 1 · senses 4

Relations on this page carry a quotation for the claim. Where a relation was separately evidenced — and so tested against the vocabulary for its type — the row says so; none of 2 relations here carry that mark. The rest are the tagger’s reading of the sentence quoted beside them.

facettagfieldconf.whose words
claim_typemethod
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claim_typethe paper's words
phenomenonauditory thalamocortical synaptic transmission
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phenomenonthe paper's words
methodelectrophysiology
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methodthe paper's words
mechanismsmechanisms
Quotation withheld — this record is not cleared for redistribution. The sentence is in the paper at the link above.
mechanisms[0]the tagger's words
mechanismsmechanisms
Quotation withheld — this record is not cleared for redistribution. The sentence is in the paper at the link above.
status: the paper's own words: it is possible that
mechanisms[1]the tagger's words
limitationsThe alternative explanation for the shell slice layer 1 sink (antidromically activated layer 5 corticotectal cells) cannot be excluded
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what this limits: the layer 1 sink interpretation: IT DOES NOT PROVE THAT THIS HYPOTHETICAL MODE OF ACTIVATION ACTUALLY OCCURS OR THAT DIRECT INPUT FROM THE NONPRIMARY AUDITORY THALAMUS HAD NO ROLE
limitations[0]the tagger's words
limitationsBecause of that unresolved alternative, the authors advise caution in using the shell preparation
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what this limits: any use of the shell preparation while the alternative is unresolved: THUS AT PRESENT IT SHOULD BE USED WITH CAUTION
limitations[1]the tagger's words
limitationsThe pharmacological design cannot isolate the AMPA/KA contribution, since APV was never given alone
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what this limits: any AMPA/KA conclusion from the pharmacology: THIS SUBTRACTIVE LOGIC CANNOT BE USED TO IMPLICATE AMPA/KA RECEPTORS BECAUSE APV WAS NEVER APPLIED BY ITSELF
limitations[2]the tagger's words
limitationsThe synaptic order of the early response is unresolved
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what this limits: the synaptic order of the early response: EXACTLY HOW MANY LINKS ARE INVOLVED IN THE EARLY RESPONSE REMAINS TO BE DETERMINED
limitations[3]the tagger's words
limitationsA measured quantity is stated not to be comparable across studies, because its visibility depends on method and criteria.
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what this limits: any comparison of IPSP amplitudes between this study and another: the authors state
limitations[4]1.0the tagger's words
limitationsThe conventional stimulation method is stated to be possibly unreliable for the purpose it is used for.
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what this limits: any result attributing a response to thalamocortical input on the basis of columnar stimulation: the authors state their MG-evoked profiles differ from those produced by conventional columnar stimulation, which may not be a reliable means of selectively activating thalamocortical inputs
limitations[5]1.0the tagger's words
limitationsTwo stimulation sites are reported not to activate the same synapses, with different laminar profiles.
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what this limits: any treatment of on-beam and MG stimulation as interchangeable probes: they are stated not to activate the same sets of synapses in cortex, and to evoke different laminar profiles
limitations[6]1.0the tagger's words
limitationsThe AMPA/KA conclusion is conditional on an assumption the authors state is difficult to prove.
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what this limits: the claim that AMPA/KA receptors mediate transmission at these synapses: it follows if one assumes the fast EPSP in the middle layers is a monosynaptic event, and the authors state it is difficult to prove that a response is monosynaptic
limitations[7]1.0the tagger's words
limitationsWhether the preparation's evoked activation resembles the intact system is stated to be undetermined.
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what this limits: any inference from these evoked responses to sound-driven cortical activity: the authors state it remains to be determined whether the activation produced by MG stimulation represents a natural pattern similar to that produced by acoustic stimulation in vivo
limitations[8]1.0the tagger's words
limitationsInhibition may be incompletely developed at the age used, indicated by the marker staining in this material.
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what this limits: any absolute inhibitory strength taken from these recordings: cortical inhibitory systems are known not to be fully developed in juvenile rodents, and the modest cortical PV labeling observed here is stated to indicate it
limitations[9]1.0the tagger's words
controlson-beam and MG-evoked IPSPs compared DIRECTLY WITHIN THE SAME SLICES rather than across the preparation
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eliminates: preparation-dependent differences - slice health, cutting angle, anything that varies between preparations and would otherwise be read as a difference between the two pathways · outcome (stated by the tagger, no quotation): held
controls[0]0.95the tagger's words
controlsa re-analysis restricted to the SEVEN CELLS in which MG-evoked EPSPs were at least as strong as on-beam EPSPs
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eliminates: weaker MG excitation as the explanation of weaker MG inhibition - if the thalamic pathway simply drove the slice less hard, smaller IPSPs would follow with no difference in inhibition itself · outcome (stated by the tagger, no quotation): held
controls[1]0.95the tagger's words
conceptscolumnar stimulation
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concepts[0]the paper's words
conceptsthalamocortical inputs
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concepts[1]the paper's words
conceptsMG stimuli
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also in: MG stimuli
concepts[2]the paper's words
conceptson-beam stimuli
Quotation withheld — this record is not cleared for redistribution. The sentence is in the paper at the link above.
concepts[3]the paper's words
conceptsIPSPs
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also in: IPSPs
concepts[4]the paper's words
conceptsexcitatory thalamic projections
Quotation withheld — this record is not cleared for redistribution. The sentence is in the paper at the link above.
concepts[5]the paper's words
conceptscortical inhibitory interneurons
Quotation withheld — this record is not cleared for redistribution. The sentence is in the paper at the link above.
concepts[6]the paper's words
sensesan IPSP amplitude, across papers - NOT A COMPARABLE QUANTITY: whether an IPSP can be observed at all, and how large it is, depends heavily on methodology and criteria, so the same named measurement in two studies is two measurements and the authors state the comparison is difficult
Quotation withheld — this record is not cleared for redistribution. The sentence is in the paper at the link above.
senses[0]1.0the tagger's words
sensescolumnar (on-beam) stimulation - NOT A SELECTIVE THALAMOCORTICAL PROBE: it is used to activate thalamocortical inputs, and the authors state it may not be a reliable means of doing so selectively, because MG and on-beam stimuli do not activate the same sets of synapses
Quotation withheld — this record is not cleared for redistribution. The sentence is in the paper at the link above.
senses[1]1.0the tagger's words
sensesthe AMPA/KA finding - HELD UP BY AN ASSUMPTION, NOT A CONTROL: the subtractive logic could not be used because APV was never applied alone, so the conclusion follows only if the fast EPSP is a monosynaptic event, which the authors state is difficult to prove
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senses[2]1.0the tagger's words
sensesthe early IPSPs - CORTICAL IN ROUTE, INFERRED FROM A BLOCKER RATHER THAN FROM THE RESPONSE: a direct inhibitory thalamic projection is ruled out because an AMPA/KA antagonist blocked them, and the remaining account is that excitatory thalamic projections drove cortical interneurons which then synapsed on the recorded cells; the paper states this as likely, not as shown
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senses[3]0.8the tagger's words
claim_relationsbuilds-on → Agmon and Connors (1991) somatosensory thalamocortical slice preparation
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claim_relations[0]the tagger's words
claim_relationsextends → Metherate and Cruikshank (1999), the authors' own initial feasibility study in rat and mouse slices
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claim_relations[1]the tagger's words
claim_relationsbuilds-on → Barth and MacDonald (1996) finding that nonlemniscal stimulation evokes cortical gamma band activity
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claim_relations[2]the tagger's words
concept_edgesMG stimuli —contrasted-with→ on-beam stimuli
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differs in: which synapses they activate, and the laminar profile they evoke · strength of the relation: 1
concept_edges[0]the tagger's words
concept_edgescortical inhibitory interneurons —causes→ IPSPs
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direction on the measure: increases · strength of the relation: 0.8
concept_edges[1]the tagger's words
content_tagst-test
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also in: t-test
content_tags.statistics[0]the tagger's words
content_tagst-test
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also in: t-test
content_tags.statistics[1]the tagger's words
content_tagsmean
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also in: mean
content_tags.statistics[2]the tagger's words
content_tagsAn in vitro mouse brain preparation can preserve functionally intact thalamocortical connections from the MGv to auditory cortex.
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content_tags.findings[0]the tagger's words
content_tagsAuditory thalamocortical transmission is carried by both AMPA/kainate and NMDA glutamate receptors.
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content_tags.findings[1]the tagger's words
content_tagsEvery primary slice analysed showed a dominant current sink in cortical layers 3-4 after medial geniculate stimulation.
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content_tags.findings[2]the tagger's words
content_tagsThalamus-evoked responses differ from those of conventional columnar stimulation, indicating columnar stimulation may not reliably activate thalamocortical inputs selectively.
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content_tags.findings[3]the tagger's words
content_tagsA second 'shell' slice is presented in which subcortical stimulation appears to selectively activate synapses in superficial layer 1 of temporal cortex.
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content_tags.findings[4]the tagger's words
content_tagsTracing confirmed that layer 5 corticotectal axons pass through the shell stimulation region on their way to the inferior colliculus.
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content_tags.findings[5]the tagger's words
content_tagson-beam stimulation
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content_tags.definitions[1]the tagger's words
content_tagsearly response
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content_tags.definitions[2]the tagger's words
content_tagslate responses
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content_tags.definitions[3]the tagger's words
content_tagsprimary slice
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also in: primary slice
content_tags.definitions[4]the tagger's words
content_tagsshell slice
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also in: shell slice
content_tags.definitions[5]the tagger's words
content_tagsstimulus intensity
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content_tags.variables[3]the tagger's words
content_tagscurrent source density
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content_tags.variables[4]the tagger's words
content_tagsamplitudes of the early responses
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content_tags.variables[5]the tagger's words
content_tags66 cells / 24 slices — number of intracellularly recorded layer 3-4 neurons and the number of primary slices they came from
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content_tags.quantities[0]the tagger's words
content_tags4.0 ± 0.1 ms — mean onset latency of the intracellular early response, statistically indistinguishable from the middle-layer CSD sink latency
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content_tags.quantities[1]the tagger's words
content_tags16/16 — proportion of histologically checked primary slices whose top contained at least part of the ventral medial geniculate
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content_tags.quantities[2]the tagger's words
content_tags15/20 — fraction of tested cells that displayed an inhibitory postsynaptic potential following the initial EPSP
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content_tags.quantities[3]the tagger's words
content_tags6.0 ± 0.2 ms — mean onset latency of the shell-slice layer 1 surface sink, longer than the infragranular sink latency
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content_tags.quantities[4]the tagger's words
content_tags22 — number of primary slices with laminar CSD analysis, all of which showed a dominant middle-layer sink
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content_tags.quantities[5]the tagger's words

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