Comprehensive Analysis of Long-Range Connectivity from and to the Posterior Parietal Cortex of the Mouse

Comprehensive Analysis of Long-Range Connectivity from and to the Posterior Parietal Cortex of the Mouse Oh Son Morris Choi Lee Rah

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Comprehensive Analysis of Long-Range Connectivity from and to the Posterior Parietal Cortex of the Mouse Cerebral Cortex 10.1093/cercor/bhaa230 Ohnd-comprehensive-analysis-of-long-range-connect Ohnd-comprehensive-analysis-of-long-range-connect.txt, the project's staged copy e28778521e503ce67c448754637319f8477fe4a4 not-redistributable neuroscience

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0 spans located by searching the stored text for the quote's exact words, allowing any whitespace between them existence Quotation withheld because this record is not cleared for redistribution. long-range connectivity of the mouse posterior parietal cortex Quotation withheld because this record is not cleared for redistribution. observation observation Quotation withheld because this record is not cleared for redistribution. mechanisms Absence of a direct visual-to-motor cortical route forces visual decision signals through PPC, so inactivating PPC impairs visual but not whisker-based decisions Quotation withheld because this record is not cleared for redistribution. mechanisms Reciprocal PPC-thalamic loops sustain persistent choice-selective firing during a delay Quotation withheld because this record is not cleared for redistribution. mechanisms Tracing and axon-volume quantification disagree because one reports where cell bodies sit and the other where synapses land Quotation withheld because this record is not cleared for redistribution. The PPC is small and its anatomical borders are weakly agreed on in mouse, so injection targeting could be imprecise. what this limits: every result reported for mouse PPC, and every join on that label: THE PPC IS A RELATIVELY SMALL BRAIN REGION WITH A WEAK CONSENSUS ON ANATOMICAL BORDERS IN THE MOUSE BRAIN Quotation withheld because this record is not cleared for redistribution. Few injections were available per structure in the atlas dataset, giving incomplete regional coverage. what this limits: the regional coverage of the atlas: A RELATIVELY LIMITED NUMBER OF INJECTIONS FOR EACH STRUCTURE ... CAN RESULT IN INCOMPLETE COVERAGE OF THE BRAIN REGIONS, WHICH MAY DISPLAY A HIGH LEVEL OF INTRAREGIONAL VARIABILITY Quotation withheld because this record is not cleared for redistribution. The quantified signal is axonal, not synaptic, so laminar projection patterns must be read cautiously. what this limits: any synaptic reading of the projection patterns: THE DETECTED SIGNAL IS PRIMARILY BASED ON AXONAL STRUCTURE INSTEAD OF SYNAPTIC INPUTS PER SE Quotation withheld because this record is not cleared for redistribution. The LD and LP injections did not fill those nuclei, so the apparent mouse-rat thalamic difference is not yet established. what this limits: the apparent mouse-rat thalamic difference: AS LD AND LP INJECTIONS ... DID NOT FILL THE ENTIRE LD OR LP, THE COMPLETE REGIONAL INJECTIONS OR ADDITIONAL NONOVERLAPPING SUBREGIONAL TARGETING ... ARE REQUIRED TO DEMONSTRATE A TRUE SPECIES DIFFERENCE Quotation withheld because this record is not cleared for redistribution. Two ways of delineating the same region are reported not to match. what this limits: any boundary assigned to mouse PPC: even with functional retinotopic mapping using intrinsic optical imaging, voltage-sensitive dyes or Ca2+ responses, the boundaries of VISa and VISrl were not clearly defined or mismatched with the architectonic definition Quotation withheld because this record is not cleared for redistribution. Injection quality varies by structure because selection falls back through three tiers. what this limits: any comparison of projection strengths between structures here: injections occupying over 90% of the primary structure were preferred, with fallback to 75-90% and then 50-75% where none passed Quotation withheld because this record is not cleared for redistribution. A printed result is withdrawn as an artefact on evidence the paper does not show. what this limits: the CA3 projection signal reported here: the authors state it appears to have resulted from a false positive quantification from the path through the ventral internal capsule Quotation withheld because this record is not cleared for redistribution. The threshold for showing a connection differs between structure classes. what this limits: any connection matrix read from this paper: only connections above threshold are shown, and the threshold is not the same for cortical and thalamic structures Quotation withheld because this record is not cleared for redistribution. injections that fill the entire LD or LP, or non-overlapping subregional targeting - NOT achieved, and the paper says the comparison cannot be made without them eliminates: an apparent mouse-versus-primate species difference in thalamic connectivity that is really a difference in where the tracer landed · outcome (stated by the tagger, no quotation): absent-declared-unreported Quotation withheld because this record is not cleared for redistribution. functional retinotopic mapping functional retinotopic mapping Quotation withheld because this record is not cleared for redistribution. architectonic definition architectonic definition Quotation withheld because this record is not cleared for redistribution. primary injection structure primary injection structure Quotation withheld because this record is not cleared for redistribution. false positive quantification false positive quantification Quotation withheld because this record is not cleared for redistribution. strong connections above threshold strong connections above threshold Quotation withheld because this record is not cleared for redistribution. the PPC's borders - TWO DELINEATIONS THAT DISAGREE: there is a weak consensus on anatomical borders, and the paper reports that functional retinotopic mapping did not clearly define the boundaries or mismatched the architectonic definition, so the region has two methods of fixing it and they do not agree Quotation withheld because this record is not cleared for redistribution. a selected injection - ONE OF THREE TIERS: preference is for injections occupying over 90% of the primary structure, falling back to 75-90% and then 50-75% where nothing passed, so the phrase 'representative injection' covers three grades of evidence and the result does not say which Quotation withheld because this record is not cleared for redistribution. a false-positive attribution - A CORRECTION WITHOUT ITS EVIDENCE: a printed projection signal is attributed to quantification of a fibre path rather than a terminal field, and the reader is asked to discount the result on that basis Quotation withheld because this record is not cleared for redistribution. builds-on → Oh et al. 2014; Harris et al. 2019 (brain-wide quantitative projection analyses) Quotation withheld because this record is not cleared for redistribution. builds-on → Zingg et al. 2017 (AAV1 transsynaptic anterograde tracing) Quotation withheld because this record is not cleared for redistribution. extends → the rat connectivity record in BAMS (Brain Architecture Management System) Quotation withheld because this record is not cleared for redistribution. contradicts → prior rat reports of a zona incerta (ZI) projection to VISrl Quotation withheld because this record is not cleared for redistribution. functional retinotopic mapping —contrasted-with→ architectonic definition functional retinotopic mapping architectonic definition differs in: where each places the boundary: the functional maps were not clearly defined or did not match the architectonic one · strength of the relation: 1 Quotation withheld because this record is not cleared for redistribution. correlation correlation Quotation withheld because this record is not cleared for redistribution. standard deviation standard deviation Quotation withheld because this record is not cleared for redistribution. mean mean Quotation withheld because this record is not cleared for redistribution. The mouse posterior parietal cortex sends its heaviest cortical output to secondary motor cortex, primary visual cortex and the barrel field of primary somatosensory cortex. Quotation withheld because this record is not cleared for redistribution. PPC output to the thalamus is concentrated in three associative nuclei, LP, PO and LD. Quotation withheld because this record is not cleared for redistribution. The heaviest inputs to the PPC come from ipsilateral secondary motor, auditory and visual cortex plus primary somatosensory cortex. Quotation withheld because this record is not cleared for redistribution. Within the hypothalamus, moderate PPC projections appeared almost exclusively in the zona incerta. Quotation withheld because this record is not cleared for redistribution. What separates PPC from neighbouring visual cortex is that it gets more multimodal spatial input and less primary visual input. Quotation withheld because this record is not cleared for redistribution. The strongest reciprocal cortical partners of PPC are MOs, SSp-bfd, VISal, VISam and VISpm. Quotation withheld because this record is not cleared for redistribution. The strongest reciprocal thalamic partners of PPC are LP, PO, LD and PF. Quotation withheld because this record is not cleared for redistribution. Neuromodulatory input to PPC is dominated by cholinergic fibres, with weaker serotonergic and histaminergic input. Quotation withheld because this record is not cleared for redistribution. The posterior thalamic nucleus projects topographically, its dorsomedial neurons reaching VISa and its lateral neurons reaching VISrl. Quotation withheld because this record is not cleared for redistribution. Compared with rats, mice have lighter LD and LP projections to PPC, while ventrobasal (VPL, VPM) and LGd input appears lightly but clearly in mice. Quotation withheld because this record is not cleared for redistribution. The greatest mouse-rat difference in PPC projection was in the infralimbic area. Quotation withheld because this record is not cleared for redistribution. Top-down cortical inputs from ILA and ACAd and higher-order thalamic input from VAL and PO terminate mainly in layer 1 of PPC. Quotation withheld because this record is not cleared for redistribution. Overall the mouse PPC connectome closely resembles the rat's, with a minority of clear species differences. Quotation withheld because this record is not cleared for redistribution. normalized projection volume normalized projection volume Quotation withheld because this record is not cleared for redistribution. injection volume injection volume Quotation withheld because this record is not cleared for redistribution. robustness of labeling (R) robustness of labeling (R) Quotation withheld because this record is not cleared for redistribution. inclusiveness of projection targets (I) inclusiveness of projection targets (I) Quotation withheld because this record is not cleared for redistribution. representative score (S) representative score (S) Quotation withheld because this record is not cleared for redistribution. reciprocality reciprocality Quotation withheld because this record is not cleared for redistribution. bilaterality bilaterality Quotation withheld because this record is not cleared for redistribution. virtual retrograde map virtual retrograde map Quotation withheld because this record is not cleared for redistribution. injection coordinates injection coordinates Quotation withheld because this record is not cleared for redistribution. projection strength projection strength Quotation withheld because this record is not cleared for redistribution. injection volume injection volume Quotation withheld because this record is not cleared for redistribution. N = 2556 — number of published atlas injection experiments that passed the standard-labelling screen and entered the analysis Quotation withheld because this record is not cleared for redistribution. 214 — unique primary injection sites, one per brain structure, from which afferents to PPC were inferred Quotation withheld because this record is not cleared for redistribution. 173 — representative injections selected for the noncortical (subcortical) structures Quotation withheld because this record is not cleared for redistribution. 0.97 ± 0.03 — Pearson correlation of the top 25 projection targets between the chosen VISp representative injection and 13 alternative injections Quotation withheld because this record is not cleared for redistribution. 0.1 for cortical and 0.05 for thalamic — normalized-projection-volume thresholds a connection had to exceed to be shown in the reciprocality analysis Quotation withheld because this record is not cleared for redistribution.

The full text of this article is not redistributable, so it is not included here. The evidence sentences are withheld with it; the tag layer and its grounding status remain. The article itself is at https://doi.org/10.1093/cercor/bhaa230.