Diode probes for spatiotemporal optical control of multiple neurons in freely moving animals

Stark, Koos, Buzsáki · Journal of Neurophysiology · 2012 · neuroscience · 10.1152/jn.00153.2012 · record Stark2012-diode-probes-for-spatiotemporal-optical-cont
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Where this record's text came from. faceted 2026-08 by reading the locally held text (html); every evidence string verified as an exact substring of that text before merging. This is a tool paper, so claim type=method and method=system-or-tool-building overlap; the in vivo validation is electrophysiological (535 hippocampal and 98 neocortical units in rats and transgenic mice) plus closed-loop behavioural experiments on a linear track, which the single method facet cannot show. The 'contradicts' relation to Aravanis et al. 2007 is a measurement discrepancy that the authors themselves explain by different testing conditions, not a rejection of that work. 2026-08-13 and 2026-08-15 schema-0.3 passes: every quote verified as an exact substring of the staged text and anchored at merge time; concept terms verified present; every edge endpoint an existing node.
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Other fields define these differently the same word, defined another way in another discipline
precision 10 fields · principal component analysis 5 fields · model 4 fields · plasticity 4 fields · standard deviation 3 fields · threshold 2 fields · and 5 more →

Tags on this paper

Every tag the record carries, each linking to every other paper in the corpus that carries it. A tag appears here whether or not its words occur in the article — the spans below are only the ones a sentence in this paper licenses. Of the 42 concept tags on this paper, none is grounded in a quoted sentence. All carry the tagger's gloss of what the term means in this paper, which is not something the article can license.
light diode laser diode fiber coupling skew rays meridional acceptance angle index reflective losses scattering wavelength model intensity threshold volume shank silicon probe photostimulation spike waveform neuron unit precision latency interneuron pyramidal ChR2 transduction cell type artifact heat closed-loop circuit network phase precession plasticity rate temporal coding limitation tether freely moving Putting the fiber tip within tens of μm of the recording sites means very little light is needed, which in turn removes the need for high currents and lets the device avoid heat sinks, optical artifacts and spike superposition while confining the effect to the monitored neurons An index-matched glue at the diode–fiber interface cuts Fresnel reflection losses and widens the fiber's acceptance angle for skew rays, which is why coupled power doubles Red-light-driven halorhodopsin hyperpolarisation of the membrane delays the ChR2-evoked spike, which is why latencies lengthen when activation and silencing are applied together The unusually high fraction of responsive units follows from the high AAV transduction rate combined with the very short distance between fiber tip and recording sites diode-probe system light-modulated unit light modulation index waveform consistency center-of-mass spiking probability closed-loop control light intensity Firing rate during light baseline period Mann-Whitney U test Wilcoxon signed-rank test Wilcoxon paired signed-rank test chi-squared test Kruskal-Wallis test exact binomial test permutation test correlation Pearson correlation principal component analysis principal component analysis (PCA) multiple-comparison correction Bonferroni correction regression robust linear regression standard deviation mean ± SD standard error mean ± SE goodness of fit U test r 2 L ratio system-or-tool-building

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relation to other workclaim_relationshow this paper stands to another: agrees, contradicts, extends, qualifies
claim typeclaim_typethe shape of the claim: existence, mechanism, method, critique, prediction, null
concept edgeconcept_edgesan asserted relation between two concepts, with the sentence asserting it
content tagcontent_tagsa finding, definition, quantity, statistic or variable named in the paper
contributioncontributionwhat the paper claims to add
controlcontrolsan experiment run to eliminate a rival explanation
limitationlimitationswhat the paper says its evidence cannot support
mechanismmechanismshow the reported effect is said to come about
methodmethodhow the paper produced its evidence
object of studyobject_of_studywhat the paper is about — the thing it investigates
phenomenonphenomenonthe effect or regularity under study
research questionresearch_questionthe question the paper sets itself
sensesensesa distinct use of a term, quoted where the paper fixes it
transfertransfersa method or concept carried in from another field

The paper

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Every tag on this page

alternatives 3 · claim_relations 10 · claim_type 1 · concept_edges 14 · content_tags 81 · contribution 1 · controls 5 · limitations 9 · mechanisms 8 · method 1 · object_of_study 1 · phenomenon 1 · research_question 1 · senses 20 · transfers 6

Relations on this page carry a quotation for the claim. Where a relation was separately evidenced — and so tested against the vocabulary for its type — the row says so; none of 14 relations here carry that mark. The rest are the tagger’s reading of the sentence quoted beside them.

facettagfieldconf.whose words
object_of_studyan instrument: independent optical control of many sites in the brain of a freely moving animal, with the stimulated neurons recorded at the same time
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object_of_studythe paper's words
research_questionhow to stimulate exactly the neurons being monitored, when electrodes hear only 60 μm and every existing light source reaches far further than that
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research_questionthe paper's words
contributiona diode-fiber assembly light enough to put one on every shank - local photostimulation and recording of the same cells, without light artifacts, spike superposition or a fiber tether
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contributionthe paper's words
claim_typemethod
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claim_typethe paper's words
phenomenonmultisite optogenetic control of neurons in freely moving animals
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phenomenonthe paper's words
methodsystem-or-tool-building
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methodthe paper's words
mechanismsmechanisms
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mechanisms[0]the tagger's words
mechanismsmechanisms
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mechanisms[1]the tagger's words
mechanismsmechanisms
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mechanisms[2]the tagger's words
mechanismsmechanisms
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mechanisms[3]the tagger's words
mechanismsthe light is weak and the fiber tip is close, so the illuminated volume is about 0.001 mm 3 - the cells the light reaches are the cells the shank can hear
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mechanisms[4]1.0the tagger's words
mechanismsa drop of index-matching glue at the diode-fiber interface, which cuts the reflective losses at each interface from 8% to under 1%
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mechanisms[5]1.0the tagger's words
mechanismsand the same glue admits skew rays from all directions, which is where the doubling in coupling efficiency actually comes from
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mechanisms[6]1.0the tagger's words
mechanismswith the light path perpendicular to the recording sites and the power several orders of magnitude below laser stimulation, so there are no local field potential artifacts to interpret
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mechanisms[7]1.0the tagger's words
limitationsThe spatial confinement of the photostimulus is itself a drawback for experiments that need a whole structure controlled synchronously.
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what this limits: the confinement that is the method's selling point: FOR SOME APPLICATIONS, THE USE OF SPATIALLY RESTRICTED PHOTOSTIMULATION FOR LOCALIZED NEURONAL CONTROL IS ACTUALLY A LIMITATION - an advantage for one use and a defect for another
limitations[0]the tagger's words
limitationsThe 1/d scattering model used to estimate light intensity overestimates power beyond 1 mm from the fiber tip.
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what this limits: every light-intensity figure beyond 1 mm from the tip: THE MODEL CONSISTENTLY OVERESTIMATED POWER AT DISTANCES >1 MM, while POWER AT SHORTER DISTANCES WAS WELL-APPROXIMATED
limitations[1]the tagger's words
limitationsThe fluorescein-bath measurement of the light cone is only an approximation of the real geometric light distribution in brain tissue.
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what this limits: the measured light cone: THIS IS ONLY A PROXY TO THE REAL GEOMETRIC DISTRIBUTION OF LIGHT IN THE BRAIN SINCE SCATTERING MAY CONTRIBUTE ... ESPECIALLY AT SHORT WAVELENGTHS
limitations[2]the tagger's words
limitationsTHE PAPER'S HEADLINE ADVANTAGE, RE-TAGGED AS A LIMITATION: spatially restricted photostimulation IS ACTUALLY A LIMITATION for experiments requiring synchronous control of an entire structure
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what this limits: experiments requiring synchronous control of an entire structure, where the spatial extent should be two to three orders of magnitude higher than the 0.001 mm 3 a shank can monitor
limitations[3]1.0the tagger's words
limitationsthe scattering model consistently overestimated power beyond 1 mm, and scattering ran 5 to 10% higher in tissue that was not fresh
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what this limits: the same scope as limitations[1], plus the tissue caveat: SCATTERING WAS SLIGHTLY HIGHER (5-10%) IN NONFRESH TISSUE
limitations[4]1.0the tagger's words
limitationsthe fluorescein image is declared A PROXY ONLY for the real geometric distribution of light in the brain, since scattering contributes to it at short wavelengths
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duplicate of: limitations[2] · what this limits: the same scope as limitations[2] - the fluorescein image stands in for the real distribution
limitations[5]1.0the tagger's words
limitationsthe high fraction of light-modulated units MAY BE DUE TO transduction rates and the proximity of the fiber tip rather than to anything general
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what this limits: the fraction of units reported as light-modulated: it MAY BE DUE TO THE HIGH ChR2 TRANSDUCTION RATES ... AND THE PROXIMITY OF THE FIBER TIP TO THE RECORDING SITES
limitations[6]1.0the tagger's words
limitationsand the count of illuminated cells rests on an assumed neuronal density and IGNORES SHADOWING by the probe shank
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what this limits: the count of illuminated cells: THE ESTIMATION ... ASSUMES NEURONAL DENSITY OF 20,000 CELLS/MM3 AND IGNORES SHADOWING BY THE PROBE SHANK
limitations[7]1.0the tagger's words
limitationswith the number of fibers a freely moving animal can be tethered to limited to one or two, which RESTRICTED the range of scientific questions that could be addressed
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what this limits: what could be asked before this method, not only what could be measured: SINCE THE NUMBER OF FIBERS A FREELY MOVING ANIMAL CAN BE TETHERED TO IS LIMITED (1-2 FOR SMALL RODENTS), THE RANGE OF SCIENTIFIC QUESTIONS THAT COULD BE ADDRESSED WAS RESTRICTED
limitations[8]1.0the tagger's words
transfersfor a uniform planar (Lambertian) light source and a straight cleaved optical fiber having a numerical aperture, the maximum coupling efficiency of Hudson 1974
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transfers[0]the tagger's words
transfersreflective losses, quantified by the Fresnel equations at each interface, and skew rays - two factors with opposing practical implications, both ignored in the standard coupling formula
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transfers[1]1.0the tagger's words
transfersa 1/d scattering model fitted to power measured through a block of brain, giving a scattering coefficient per wavelength
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transfers[2]1.0the tagger's words
transfersred laser diodes driven by similar currents, yielding light at the fiber tip that subtended a similar illuminated volume of brain tissue
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transfers[3]1.0the tagger's words
transferswaveguides embedded in the probe shank, named as the route to scaling the number of sources
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transfers[4]0.9the tagger's words
transfersclosed-loop triggering - the recorded signal decides when the stimulus happens
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transfers[5]1.0the tagger's words
controlsspike waveforms during light compared with waveforms during spontaneous activity
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eliminates: the light distorting or superimposing the spikes it evokes - median waveform consistencies 0.977 against 0.983, U test P = 0.19 · outcome (stated by the tagger, no quotation): held
controls[0]1.0the tagger's words
controlsunits recorded on the shanks that were NOT illuminated, 200 μm and further away
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eliminates: the effect spreading beyond the illuminated shank - 52% modulated on the lit shank against 6.8% nearby, at chance level by an exact binomial test · outcome (stated by the tagger, no quotation): held
controls[1]1.0the tagger's words
controlsthe same cross-correlation histograms computed over a duration-matched baseline period before any stimulation
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eliminates: the correlations being native rather than induced - the baseline histograms were nearly flat WITH AN EXCEPTION, a 1-ms lag between the units on shanks 4 and 3 · outcome (stated by the tagger, no quotation): partial
controls[2]1.0the tagger's words
controlsthe wideband traces inspected for photoelectrical artifacts at the light powers actually used
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eliminates: light artifacts masquerading as physiology - the weak stimulus produced no LFP artifacts, unlike powers three orders of magnitude higher · outcome (stated by the tagger, no quotation): held
controls[3]1.0the tagger's words
controlsthe scattering fit repeated across tip morphology, source power and brain region
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eliminates: the attenuation constant depending on how it was measured - it did not, EXCEPT that scattering was 5 to 10% higher in nonfresh tissue and the model overestimates beyond 1 mm · outcome (stated by the tagger, no quotation): partial
controls[4]1.0the tagger's words
sensesTHE PAPER'S HEADLINE ADVANTAGE, RE-TAGGED AS A LIMITATION BY ITS OWN AUTHORS: for some applications the use of SPATIALLY RESTRICTED PHOTOSTIMULATION for localized neuronal control IS ACTUALLY A LIMITATION - where synchronous control of an entire structure is wanted, the spatial extent should be two to three ORDERS OF MAGNITUDE HIGHER than the 0.001 mm 3 that can be monitored
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senses[0]1.0the tagger's words
sensesand the resolution is not a better setting but a different question - THE SPECIFIC LIGHT SOURCES AND FIBERS SHOULD BE TAILORED TO THE SCIENTIFIC QUESTION AT HAND
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senses[1]1.0the tagger's words
sensesONE INTERVENTION, TWO COMPONENTS, OPPOSITE SIGNS: the index-matching glue reduces reflective losses considerably, to under 1%, BUT AT A COST OF REDUCING the effective acceptance angle of the fiber BY 41%
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senses[2]1.0the tagger's words
sensesthe same glue on the other component: its effect on the SKEW RAYS acceptance angle is DIAMETRICALLY OPPOSITE from its effect on the meridional one, enabling acceptance from all directions and A TWOFOLD INCREASE in coupling efficiency - the net sign is empirical, not derivable
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senses[3]1.0the tagger's words
senseswith the two ignored factors named as ignored: TWO FACTORS, WHICH HAVE OPPOSING PRACTICAL IMPLICATIONS, WERE IGNORED IN THE FOREGOING - reflective losses, and skew rays
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senses[4]1.0the tagger's words
sensesA MEASURED CONSTANT THAT DISAGREES WITH THE PUBLISHED ONE, AND THE DISAGREEMENT DECLARED NOT TO MATTER: blue-light scattering 7.18 against a previously reported 10.3 for rat brain, explained by the slightly different testing conditions - and IN ANY EVENT estimates were SIMILAR REGARDLESS OF THE PRECISE NUMBER USED
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senses[5]1.0the tagger's words
sensesthe model's own failure stated with its range: it CONSISTENTLY OVERESTIMATED power beyond 1 mm while fitting well below it, and scattering was 5 to 10% higher in nonfresh tissue
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senses[6]1.0the tagger's words
sensesand the empirical map declared a proxy: THIS IS ONLY A PROXY to the real geometric distribution of light in the brain, since scattering may contribute, ESPECIALLY AT SHORT WAVELENGTHS
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senses[7]1.0the tagger's words
sensesWHY THE LIGHT HAS TO BE WEAK AND CLOSE: electrodes can only disambiguate spikes of neurons up to 60 μm away, so stimulating from the surface or from a fiber a few hundred μm off INEVITABLY ACTIVATES MANY MORE UNMONITORED NEURONS THAN ARE BEING MONITORED, MAKING THE SEPARATION BETWEEN DIRECT AND POPULATION-MEDIATED EFFECTS IMPOSSIBLE
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senses[8]1.0the tagger's words
sensesand strong light of ANY wavelength carries energy dissipated as HEAT, which has a MIXED DE-/HYPERPOLARIZING effect - a confound that does not depend on the opsin at all
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senses[9]1.0the tagger's words
sensesTHE SAME LIGHT, THE OPPOSITE SIGN, DECIDED BY THE PROMOTER: identical parameters in CA1 give robust spiking of pyramidal cells in CaMKII::ChR2 mice, and in PV::ChR2 mice spiking of interneurons FOLLOWED BY SILENCING of the nearby pyramidal cells
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senses[10]1.0the tagger's words
sensesA 25% CHANGE IN DOSE FLIPS THE OUTCOME: 70% of the 0.35 mW/mm 2 blue pulses overcame the red-light hyperpolarization but ONLY 10% of the 0.28 mW/mm 2 pulses did - the same two opsins, the same cells, and a sharp threshold between activation and silencing
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senses[11]1.0the tagger's words
sensesa statistical definition rescued by the data's own shape: the definition of light-modulated spiking WAS STATISTICAL, but the modulation indices exhibited a BIMODAL distribution, 0.045 against 0.76
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senses[12]1.0the tagger's words
senseswith the fraction itself hedged - the relatively high fraction MAY BE DUE TO high transduction rates and the proximity of the fiber tip
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senses[13]1.0the tagger's words
sensesTHE UNIT IS DEFINED BY THREE NUMBERED GATES BEFORE ANY RESULT IS COMPUTED: peak-to-peak amplitude above 50 microvolts, L ratio below 0.05, and a clear refractory period - and then a fourth for the statistics, at least 100 spikes above 0.01 spikes/s
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senses[14]1.0the tagger's words
sensesan idealisation declared inside a figure legend: the estimate ASSUMES neuronal density of 20,000 cells/mm 3 and IGNORES SHADOWING by the probe shank
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senses[15]1.0the tagger's words
sensesthe temporal resolution stated as three numbers that mean different things - center-of-mass 12 ms, latency 5.5 ms, and precision, the across-trial SD, 2 ms
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senses[16]1.0the tagger's words
sensesAND THE INSTRUMENT'S PURPOSE IS A DEBATE: spatiotemporally sequenced activity at different timescales COULD ADDRESS THE DEBATE ON RATE VS. TEMPORAL CODING - the same debate Levi2022 would later call rooted in definitions
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senses[17]1.0the tagger's words
sensesand the synthetic pattern is taken up by the native one: units driven by nonperiodic photostimuli exhibited PHASE PRECESSION, indicating that NATIVE NETWORK DYNAMICS CAN INCORPORATE SYNTHETIC PATTERNS OF ACTIVITY
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senses[18]1.0the tagger's words
senseswith the constraint that motivated the whole device stated as a number: the number of fibers a freely moving animal can be tethered to is ONE TO TWO for small rodents, and that RESTRICTED THE RANGE OF SCIENTIFIC QUESTIONS THAT COULD BE ADDRESSED
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senses[19]1.0the tagger's words
alternativesclaim_type: method
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a second reading reaching the same value. Recorded: method — note: a second reading reaching the same value from a different sentence. Not applied - a person decides.
alternatives.claim_typethe tagger's words
alternativesmethod: couple a miniature diode to a small-core fiber etched to a point, glue it 50 μm above the top recording site, and drive each diode from its own precision current channel
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a second reading DIFFERING from the recorded value. Recorded: system-or-tool-building — note: a second reading, verbatim-verified, disagreeing with the value on the record. Not applied - a person decides.
alternatives.methodthe tagger's words
alternativesphenomenon: light-evoked spiking in intact tissue, and how far in space and time the evoked spikes stay where they were aimed
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a second reading DIFFERING from the recorded value. Recorded: multisite optogenetic control of neurons in freely moving animals — note: a second reading, verbatim-verified, disagreeing with the value on the record. Not applied - a person decides.
alternatives.phenomenonthe tagger's words
claim_relationsextends → Royer et al. 2010 (small-core fibers coupled to DPSS lasers at low power)
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claim_relations[0]the tagger's words
claim_relationscontradicts → Aravanis et al. 2007 (published blue-light scattering coefficient for rat brain)
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claim_relations[1]the tagger's words
claim_relationsbuilds-on → Fujisawa et al. 2008 (probe implantation methods)
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claim_relations[2]the tagger's words
claim_relationscontradicts → the previously reported blue-light scattering coefficient for rat brain, 10.3, against 7.18 measured here
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differs in: light was shined through brain slices in the previous measurements, which causes reflective losses
claim_relations[3]1.0the tagger's words
claim_relationscritiques → stimulation from the brain surface or from large fibers hundreds of μm away, which activates far more neurons than are monitored
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differs in: THE AXIS IS THE MISMATCH BETWEEN THE RECORDED AND THE ILLUMINATED VOLUME. 'such electrodes can only record and disambiguate spikes of nearby (UP TO 60 UM) NEURONS, [so] stimulation of continuously monitored cells requires placing the light source very close to the recording site.' The recording radius is given as a number and the stimulated volume is larger - so a causal claim from optogenetics rests on cells that were driven and never observed.
claim_relations[4]1.0the tagger's words
claim_relationscritiques → large-core fibers driven by strong light sources, which add heat, photoelectrical artifacts and synchronous activation
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differs in: THE AXIS IS HEAT AS A CONFOUND OF THE LIGHT ITSELF. 'Quotation withheld.' The control for an optogenetic manipulation is light, and light is not inert - the confound is a property of the energy delivered, so it is present in every experiment of this kind at sufficient power.
claim_relations[5]1.0the tagger's words
claim_relationsdistinguishes-from → small-core fibers coupled to lasers at low power, which solved the artifact problem but not the tether limit of one to two fibers
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differs in: THE AXIS IS THE TETHER LIMIT, GIVEN AS A NUMBER. 'THESE ISSUES WERE PREVIOUSLY ADDRESSED IN PART BY COUPLING SMALL-CORE FIBERS TO DPSS LASERS DRIVEN AT LOW POWER, BUT SINCE THE NUMBER OF FIBERS A freely moving animal can be tethered to is limited (1-2 FOR SMALL RODENTS), the range of scientific questions that could be addressed was restricted.' The earlier solution is credited with fixing the artifact and charged with a different limit - so the two are not better and worse, they fail at different points.
claim_relations[6]1.0the tagger's words
claim_relationssupports → coincidence between the illuminated volume and the volume a silicon probe shank can monitor, as the condition for separating direct from population-mediated effects
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claim_relations[7]1.0the tagger's words
claim_relationspredicts → that MEMS integration of diode dies with waveguides in the shank will scale up the number of light sources
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claim_relations[8]0.9the tagger's words
claim_relationsqualifies → its own central advantage, which is a limitation whenever the question needs an entire structure driven at once
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differs in: THE AXIS IS WHETHER THE QUESTION NEEDS A WHOLE STRUCTURE DRIVEN AT ONCE. 'Quotation withheld.' The paper's own headline advantage is a defect for a class of question, said by the authors - so 'higher spatial precision' is not an improvement, it is a trade whose sign depends on the experiment.
claim_relations[9]1.0the tagger's words
concept_edgesphotostimulation —is-a→ limitation
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strength of the relation: 1 · note: THE HEADLINE ADVANTAGE RE-TAGGED AS A LIMITATION BY ITS OWN AUTHORS - spatially restricted photostimulation IS ACTUALLY A LIMITATION for experiments requiring synchronous control of an entire structure Audit 2026-09-28: DOUBTFUL - read against its span and kept, but the span calls spatially restricted photostimulation a limitation for some uses; is-a is loose.
concept_edges[3]1.0the tagger's words
concept_edgesfiber —causes→ volume
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strength of the relation: 1 · note: and the choice is not a better setting but a different question - THE SPECIFIC LIGHT SOURCES AND FIBERS SHOULD BE TAILORED TO THE SCIENTIFIC QUESTION AT HAND Audit 2026-09-28: DOUBTFUL - read against its span and kept, but multiple fibers can illuminate a large volume; causes is loose.
also in: fiber · volume
concept_edges[5]1.0the tagger's words
concept_edgesreflective losses —causes→ coupling
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strength of the relation: 1 · note: TWO FACTORS, WHICH HAVE OPPOSING PRACTICAL IMPLICATIONS, WERE IGNORED IN THE FOREGOING - reflective losses, quantified by the Fresnel equations at 8%, and skew rays Audit 2026-09-28: DOUBTFUL - read against its span and kept, but reflective losses and skew rays have opposing implications for coupling; causes is loose and unsigned.
concept_edges[6]1.0the tagger's words
concept_edgesskew rays —causes→ coupling
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strength of the relation: 1 Audit 2026-09-28: DOUBTFUL - read against its span and kept, but reflective losses and skew rays have opposing implications for coupling; causes is loose and unsigned.
also in: skew rays · coupling
concept_edges[7]1.0the tagger's words
concept_edgesindex —causes→ reflective losses
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strength of the relation: 1 Audit 2026-09-28: DOUBTFUL - read against its span and kept, but a matched coupling index reduces reflective losses; the edge carries no direction.
concept_edges[8]1.0the tagger's words
concept_edgesindex —causes→ acceptance angle
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strength of the relation: 1 · note: ONE INTERVENTION, OPPOSITE SIGNS - the glue reduces reflective losses to under 1% BUT AT A COST OF REDUCING the effective acceptance angle BY 41% Audit 2026-09-28: DOUBTFUL - read against its span and kept, but the span relates the coupling index to the acceptance angle; causes is loose.
concept_edges[9]1.0the tagger's words
concept_edgesskew rays —contrasted-with→ meridional
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strength of the relation: 1 Audit 2026-09-28: DOUBTFUL - read against its span and kept, but skew rays versus meridional rays; contrasted-with is implied.
also in: skew rays · meridional
concept_edges[11]1.0the tagger's words
concept_edgeslight —causes→ artifact
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strength of the relation: 1 · note: the high intensity used for deep neurons may generate superposition of multiple spike waveforms and CONSIDERABLE LIGHT ARTIFACTS Audit 2026-09-28: DOUBTFUL - read against its span and kept, but the span discusses artifacts from light near electrodes; causes is loose.
also in: light · artifact
concept_edges[15]1.0the tagger's words
concept_edgeslight —causes→ heat
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strength of the relation: 1 · note: strong light OF ANY WAVELENGTH carries considerable energy dissipated as heat - a confound that does not depend on the opsin
also in: light · heat
concept_edges[18]1.0the tagger's words
concept_edgesheat —causes→ neuron
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strength of the relation: 1 · note: local heating has a MIXED DE-/HYPERPOLARIZING effect, which may confound and/or limit the effect of the optogenetic manipulation itself Audit 2026-09-28: DOUBTFUL - read against its span and kept, but local heating can affect neurons; causes is loose.
also in: heat · neuron
concept_edges[19]1.0the tagger's words
concept_edgesphotostimulation —causes→ rate
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strength of the relation: 1 · note: a statistical definition rescued by the data's own shape - the definition WAS STATISTICAL, but the indices were BIMODAL, 0.045 against 0.76 Audit 2026-09-28: DOUBTFUL - read against its span and kept, but light modulation indices compare firing during photostimulation with baseline; causes 'rate' is terse.
concept_edges[29]1.0the tagger's words
concept_edgesinterneuron —causes→ pyramidal
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strength of the relation: 1 · note: THE SAME LIGHT, THE OPPOSITE SIGN, DECIDED BY THE PROMOTER - identical parameters give pyramidal spiking in CaMKII::ChR2 and, in PV::ChR2, interneuron spiking FOLLOWED BY SILENCING of nearby pyramidal cells Audit 2026-09-28: DOUBTFUL - read against its span and kept, but PV-driven interneurons silenced nearby pyramidal cells; causes 'pyramidal' drops the silencing.
concept_edges[33]1.0the tagger's words
concept_edgesrate —contrasted-with→ temporal coding
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strength of the relation: 1 · note: THE INSTRUMENT'S PURPOSE IS A DEBATE - the same debate Levi2022 would later call ROOTED IN DEFINITIONS Audit 2026-09-28: DOUBTFUL - read against its full span and kept, but the span names the 'debate on rate vs. temporal coding'; contrasted-with is attributed.
also in: rate · temporal coding
concept_edges[40]1.0the tagger's words
concept_edgesdiode —part-of→ silicon probe
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strength of the relation: 1 · note: and the stated next step is to stop gluing - MEMS integration of diode dies with waveguides embedded IN the shanks Audit 2026-09-28: DOUBTFUL - read against its full span and kept, but MEMS 'can be used to' integrate diode dies with a silicon probe's shanks; part-of is prospective.
also in: diode · silicon probe
concept_edges[41]1.0the tagger's words
content_tagsMann-Whitney U test
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content_tags.statistics[0]the tagger's words
content_tagsWilcoxon signed-rank test
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content_tags.statistics[1]the tagger's words
content_tagschi-squared test
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content_tags.statistics[2]the tagger's words
content_tagsKruskal-Wallis test
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content_tags.statistics[3]the tagger's words
content_tagsexact binomial test
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content_tags.statistics[4]the tagger's words
content_tagspermutation test
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content_tags.statistics[5]the tagger's words
content_tagscorrelation
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also in: correlation
content_tags.statistics[6]the tagger's words
content_tagsprincipal component analysis
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content_tags.statistics[7]the tagger's words
content_tagsmultiple-comparison correction
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content_tags.statistics[8]the tagger's words
content_tagsregression
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also in: regression
content_tags.statistics[9]the tagger's words
content_tagsstandard deviation
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content_tags.statistics[10]the tagger's words
content_tagsstandard error
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content_tags.statistics[11]the tagger's words
content_tagsgoodness of fit
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content_tags.statistics[12]the tagger's words
content_tagsU test
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also in: U test
content_tags.statistics[13]1.0the tagger's words
content_tagsexact binomial test
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content_tags.statistics[14]1.0the tagger's words
content_tagspermutation test
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content_tags.statistics[15]1.0the tagger's words
content_tagsr 2
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also in: r 2
content_tags.statistics[16]1.0the tagger's words
content_tagsL ratio
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also in: L ratio
content_tags.statistics[17]1.0the tagger's words
content_tagsWeak blue light delivered through the fiber-coupled LEDs drove 42% of recorded hippocampal units (57 of 135) to change their firing rate in freely moving rats.
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content_tags.findings[0]the tagger's words
content_tagsspike waveforms during light and during spontaneous activity were SIMILAR - median waveform consistencies 0.977 for light-modulated and 0.983 for nonresponsive units, U test P = 0.19
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content_tags.findings[1]the tagger's words
content_tagsunits recorded on the illuminated shank were often modulated, 20/39 units or 52%, whereas the fraction of modulated units on other shanks was AT CHANCE LEVEL
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content_tags.findings[2]the tagger's words
content_tagsLight-evoked spiking was temporally precise, with a median across-trial jitter of 2 ms.
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content_tags.findings[3]the tagger's words
content_tagsBlue light with identical parameters in CA1 of PV::ChR2 mice drove interneurons to spike and then silenced nearby pyramidal cells.
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content_tags.findings[4]the tagger's words
content_tagsreliable activation of units on shank 1 during blue light pulses and robust silencing of THE SAME UNITS during the red light pulses
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content_tags.findings[5]the tagger's words
content_tagsAn index-matching coupling medium at the diode-fiber interface doubled the light output, to 30-50 microwatts for blue LEDs.
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content_tags.findings[6]the tagger's words
content_tagsthese units exhibited phase precession
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content_tags.findings[7]the tagger's words
content_tagsthe mean scattering coefficients depended on wavelength
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content_tags.findings[8]the tagger's words
content_tagsthe fraction of significantly light-modulated units exhibited a marked increase between 3.5 and 5.5 mW/mm 2, from 15 to 73%
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content_tags.findings[9]the tagger's words
content_tagsTHE PAPER'S HEADLINE ADVANTAGE, RE-TAGGED AS A LIMITATION: spatially restricted photostimulation IS ACTUALLY A LIMITATION where synchronous control of an entire structure is wanted
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content_tags.findings[10]1.0the tagger's words
content_tagsand the resolution is a question, not a setting - THE SPECIFIC LIGHT SOURCES AND FIBERS SHOULD BE TAILORED TO THE SCIENTIFIC QUESTION AT HAND
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content_tags.findings[11]1.0the tagger's words
content_tagsONE INTERVENTION, OPPOSITE SIGNS: the index-matching glue cuts reflective losses to under 1% BUT AT A COST OF REDUCING the acceptance angle BY 41%
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content_tags.findings[12]1.0the tagger's words
content_tagswhile its effect on the skew rays acceptance angle is DIAMETRICALLY OPPOSITE, giving A TWOFOLD INCREASE in coupling efficiency
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content_tags.findings[13]1.0the tagger's words
content_tagsA MEASURED CONSTANT DISAGREEING WITH THE PUBLISHED ONE: blue-light scattering 7.18 against a reported 10.3, and IN ANY EVENT the estimates were SIMILAR REGARDLESS OF THE PRECISE NUMBER USED
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content_tags.findings[14]1.0the tagger's words
content_tagsthe fitted model CONSISTENTLY OVERESTIMATED power beyond 1 mm and ran 5 to 10% off in nonfresh tissue
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content_tags.findings[15]1.0the tagger's words
content_tagsWHY THE LIGHT MUST BE WEAK AND CLOSE: stimulating further away INEVITABLY ACTIVATES MANY MORE UNMONITORED NEURONS THAN ARE BEING MONITORED, MAKING THE SEPARATION BETWEEN DIRECT AND POPULATION-MEDIATED EFFECTS IMPOSSIBLE
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content_tags.findings[16]1.0the tagger's words
content_tagsand strong light of ANY wavelength adds HEAT with a MIXED DE-/HYPERPOLARIZING effect, a confound independent of the opsin
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content_tags.findings[17]1.0the tagger's words
content_tagsTHE SAME LIGHT, THE OPPOSITE SIGN, DECIDED BY THE PROMOTER: pyramidal cells spike in CaMKII::ChR2 mice and are SILENCED in PV::ChR2 mice under identical parameters
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content_tags.findings[18]1.0the tagger's words
content_tagsA 25% CHANGE IN DOSE FLIPS THE OUTCOME: 70% of 0.35 mW/mm 2 pulses efficient against 10% of 0.28 mW/mm 2 pulses
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content_tags.findings[19]1.0the tagger's words
content_tagsthe statistical definition of light modulation is rescued by a BIMODAL distribution of the indices, 0.045 against 0.76
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content_tags.findings[20]1.0the tagger's words
content_tagswith the response fraction hedged - it MAY BE DUE TO transduction rates and the proximity of the fiber tip
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content_tags.findings[21]1.0the tagger's words
content_tagsTHE UNIT IS DEFINED BY NUMBERED GATES BEFORE ANY RESULT: amplitude, L ratio, refractory period, then a firing floor that cut 535 hippocampal units to 135
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content_tags.findings[22]1.0the tagger's words
content_tagsan idealisation declared inside a figure legend - ASSUMES neuronal density of 20,000 cells/mm 3 and IGNORES SHADOWING by the probe shank
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content_tags.findings[23]1.0the tagger's words
content_tagsthe empirical light map is declared A PROXY ONLY, since scattering contributes ESPECIALLY AT SHORT WAVELENGTHS
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content_tags.findings[24]1.0the tagger's words
content_tagsNATIVE NETWORK DYNAMICS CAN INCORPORATE SYNTHETIC PATTERNS OF ACTIVITY - units driven by nonperiodic photostimuli exhibited PHASE PRECESSION
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content_tags.findings[25]1.0the tagger's words
content_tagsand the whole device exists to remove one number - a freely moving animal can be tethered to ONE OR TWO fibers, which RESTRICTED the questions that could be addressed
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content_tags.findings[26]1.0the tagger's words
content_tagsdiode-probe system
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content_tags.definitions[0]the tagger's words
content_tagslight-modulated unit
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content_tags.definitions[1]the tagger's words
content_tagslight modulation index
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content_tags.definitions[2]the tagger's words
content_tagswaveform consistency
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content_tags.definitions[3]the tagger's words
content_tagscenter-of-mass
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content_tags.definitions[4]the tagger's words
content_tagsspiking probability
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content_tags.definitions[5]the tagger's words
content_tagsclosed-loop control
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content_tags.definitions[6]the tagger's words
content_tagsthreshold
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also in: threshold
content_tags.definitions[7]1.0the tagger's words
content_tagscoupling
Quotation withheld — this record is not cleared for redistribution. The sentence is in the paper at the link above.
also in: coupling
content_tags.definitions[8]1.0the tagger's words
content_tagsunit
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also in: unit
content_tags.definitions[9]1.0the tagger's words
content_tagsprecision
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also in: precision
content_tags.definitions[10]0.9the tagger's words
content_tagslight intensity
Quotation withheld — this record is not cleared for redistribution. The sentence is in the paper at the link above.
content_tags.variables[0]the tagger's words
content_tagsFiring rate during light
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content_tags.variables[1]the tagger's words
content_tagsspiking probability
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content_tags.variables[2]the tagger's words
content_tagsbaseline period
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content_tags.variables[5]the tagger's words
content_tagsintensity
Quotation withheld — this record is not cleared for redistribution. The sentence is in the paper at the link above.
also in: intensity
content_tags.variables[6]1.0the tagger's words
content_tagsprecision
Quotation withheld — this record is not cleared for redistribution. The sentence is in the paper at the link above.
also in: precision
content_tags.variables[7]1.0the tagger's words
content_tagsrate
Quotation withheld — this record is not cleared for redistribution. The sentence is in the paper at the link above.
also in: rate
content_tags.variables[8]1.0the tagger's words
content_tagscoupling
Quotation withheld — this record is not cleared for redistribution. The sentence is in the paper at the link above.
also in: coupling
content_tags.variables[9]1.0the tagger's words
content_tags42%; 57/135 — Fraction and count of hippocampal units whose firing rate was modulated by weak blue light
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content_tags.quantities[0]the tagger's words
content_tagsmedian, 2 ms — Across-trial standard deviation of spike centers-of-mass, i.e. the temporal precision of light-evoked spiking
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content_tags.quantities[1]the tagger's words
content_tagsmedian, 5.5 ms — Median latency from light onset to the first significantly elevated PSTH bin
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content_tags.quantities[2]the tagger's words
content_tags∼0.001 mm 3 — Volume of brain tissue illuminated above the ChR2 activation threshold by a single fiber
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content_tags.quantities[3]the tagger's words
content_tags20–100 — Estimated number of neurons inside the illuminated volume, given assumed cortical cell density
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content_tags.quantities[4]the tagger's words
content_tags0.1–0.15% — Diode-to-fiber coupling efficiency for blue LEDs before index matching, versus about 2.5% for laser diodes
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content_tags.quantities[5]the tagger's words
content_tags100–130 mg — Weight of a single diode-fiber assembly, which is what keeps the headgear light enough for free movement
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content_tags.quantities[6]the tagger's words
content_tags0.5–1.5 g — Weight of a complete implantation-ready multidiode device
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content_tags.quantities[7]the tagger's words
content_tags535 hippocampal and 98 neocortical units — Total number of single units isolated that met the amplitude and isolation criteria
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content_tags.quantities[8]the tagger's words
content_tags1.82 — Median ratio of spiking probability during blue light to duration-matched spontaneous periods
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content_tags.quantities[9]the tagger's words
content_tags70% ... 10% — Fraction of blue pulses able to break through halorhodopsin silencing at 0.35 versus 0.28 mW/mm2, showing a sharp intensity threshold
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content_tags.quantities[10]the tagger's words
content_tags0.001 mm 3 — the illuminated volume, and the paper's unit of claim - the tissue a shank of a silicon probe can also monitor
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content_tags.quantities[11]1.0the tagger's words
content_tags7.18 ± 0.17 — the scattering coefficient measured for blue light, against a previously reported 10.3 for rat brain
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content_tags.quantities[12]1.0the tagger's words
content_tags41% — the acceptance angle the index-matching glue COSTS, at the same time as it removes the reflective losses
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content_tags.quantities[13]1.0the tagger's words
content_tags2 ms — the temporal precision of light-evoked spiking, the across-trial SD of the centers-of-mass
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content_tags.quantities[14]1.0the tagger's words
content_tags70% — the fraction of 0.35 mW/mm 2 blue pulses that overcame the red-light hyperpolarization, against 10% at 0.28
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content_tags.quantities[15]1.0the tagger's words
content_tags20,000 cells/mm 3 — the neuronal density ASSUMED by the illuminated-cell estimate, in a legend that also says it ignores shadowing
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content_tags.quantities[16]1.0the tagger's words

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© 2026 Shir Sivroni · ORCID 0009-0000-2597-2824