Functional organization of mouse auditory cortex in response to stimulus complexity and brain state

Afrashteh, Jafari, Sun, Kyweriga, Mohajerani · 2022 · neuroscience · 10.1101/2022.08.11.503675 · record afrashteh2022-functional-organization-of-mouse-a
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Where this record's text came from. faceted 2026-08 by reading the locally held text (pdftext); every evidence string verified as an exact substring of that text before merging. Replication status is 'untested' in the external sense: no independent replication is reported. The paper does demonstrate internal reproducibility of its maps within a session, across three weeks, and between animals ('These maps are highly replicable across weeks and between animals.'), which is a within-study consistency check rather than a replication of a prior finding. method uses a label outside the suggested list because wide-field mesoscale calcium imaging is neither electrophysiology nor fMRI. This is a bioRxiv preprint, not peer reviewed, as the header stamped on every page states. State effects reported include better high-frequency sensitivity awake and shrunken fast-FM regions under anaesthesia ('In the awake versus anesthetized state, the amplitude of tonotopic maps was greater in response to high frequencies (i.e., better hearing sensitivity).'). 2026-08-14 and 2026-08-15 schema-0.3 passes: every quote verified as an exact substring of the staged text and anchored at merge time; concept terms verified present; every edge endpoint an existing node.
How to read this page. The article itself is not reproduced here — this paper is not cleared for redistribution, so what follows is the tag layer alone. The evidence sentences are withheld with the article. The table at the foot lists every tag the record carries; each row names the facet it belongs to, and the whose words column says whether the tag records the paper's own wording or the tagger's sentence about the paper. Every tag links to each other paper in the corpus carrying it. The machine-readable edition of the same thing is the TEI file beside this one, where the tags live in standOff, and the article is at its publisher via the link below. When we have changed our mind. Some entries in the table begin with a word in capitals and the reason for it. EVIDENCE CORRECTED — the finding still stands, but we had quoted the wrong sentence from the paper, and the quotation has been replaced with the right one. RETRACTED — the entry itself was wrong; it stays on the page, marked, with the reason, rather than being deleted. WITHDRAWN AND REPLACED — the entry was wrong, and a corrected version of it appears in the entries below. Each mark carries its own reason, in brackets, because the reasons differ from one entry to the next. An entry we have WITHDRAWN is removed from this page and kept in the record, with its reason. A line reading Audit and a date is a later re-reading of the entry against its whole sentence: DOUBTFUL means it was kept, and the reason says what is loose about it. Quotation marks inside the tagger's sentence. Where a tag is the tagger's own sentence, a phrase in quotation marks inside it is borrowed from the paper — the tagger's wording around it is not. The paper's own sentence is the evidence quote for that row; on this page it is withheld with the article, and the article is at its publisher. Nothing inside quotation marks anywhere on this page is ours: if it is quoted, it is the paper's. Whose words a concept carries. A concept entry records a term the paper itself uses, so the term, the sense given for it, and its evidence are the paper's own words, quoted where the paper fixes them. This is the opposite of a sense entry in the tag table, which is the tagger's sentence about how the paper uses a term. Where a concept entry could not be grounded in the paper's words, the entry says so on its own row.
Other fields define these differently the same word, defined another way in another discipline
AI 8 fields · standard deviation 3 fields · bootstrap 2 fields

Tags on this paper

Every tag the record carries, each linking to every other paper in the corpus that carries it. A tag appears here whether or not its words occur in the article — the spans below are only the ones a sentence in this paper licenses. Of the 4 concept tags on this paper, all 4 are grounded in a quoted sentence.
tonotopic organization UF AI auditory cortex Isoflurane reduces cochlear blood flow, blocks acetylcholine receptors and enhances GABA-gated currents, which raises medial olivocochlear efferent inhibition and so depresses auditory sensitivity in the anesthetized state Behaviourally relevant high frequencies are over-represented in the mouse auditory cortex because the mouse hearing range extends much further upward, which is why the newly found regions are all high-frequency ones core auditory area belt auditory region frequency sweep rate V/T ratio responsive pixel tone frequency and sound pressure level best frequency pixel responsiveness body temperature sound pressure level frequency coverage sweep direction sweep duration Kruskal-Wallis test Kruskal-Wallis test at each pixel chi-squared goodness-of-fit test Chi-square goodness-of-fit test bootstrap the bootstrap method Wilcoxon rank-sum test one-sided Wilcoxon rank-sum test p-value standard error of the mean standard error of the mean (SEM) regression linear ridge regression coefficient of determination explained variance singular value decomposition singular value decomposition (SVD) standard deviation calcium-imaging
Method, as described: wide-field-calcium-imaging

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relation to other workclaim_relationshow this paper stands to another: agrees, contradicts, extends, qualifies
claim typeclaim_typethe shape of the claim: existence, mechanism, method, critique, prediction, null
concept edgeconcept_edgesan asserted relation between two concepts, with the sentence asserting it
conceptconceptsan idea the paper works with, quoted where the paper uses it
content tagcontent_tagsa finding, definition, quantity, statistic or variable named in the paper
controlcontrolsan experiment run to eliminate a rival explanation
limitationlimitationswhat the paper says its evidence cannot support
mechanismmechanismshow the reported effect is said to come about
methodmethodhow the paper produced its evidence
phenomenonphenomenonthe effect or regularity under study
sensesensesa distinct use of a term, quoted where the paper fixes it

The paper

The full text is not reproduced here. This article is not cleared for redistribution, so the page carries the tag layer only. Its evidence sentences are withheld; the grounding status remains with each tag, and the article itself is at https://doi.org/10.1101/2022.08.11.503675. The reading view exists for the papers whose licence permits it.

Every tag on this page

claim_relations 4 · claim_type 1 · concept_edges 1 · concepts 4 · content_tags 43 · controls 2 · limitations 7 · mechanisms 2 · method 1 · phenomenon 1 · senses 4

Relations on this page carry a quotation for the claim. Where a relation was separately evidenced — and so tested against the vocabulary for its type — the row says so; none of 1 relation here carry that mark. The rest are the tagger’s reading of the sentence quoted beside them.

facettagfieldconf.whose words
claim_typeexistence
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claim_typethe paper's words
phenomenontonotopic and FM-sweep-rate maps in mouse auditory cortex
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phenomenonthe paper's words
methodcalcium-imaging
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methodthe paper's words
mechanismsmechanisms
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mechanisms[0]the tagger's words
mechanismsmechanisms
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mechanisms[1]the tagger's words
limitationsImaging was confined to the right auditory cortex, so lateralization of vocal processing could not be assessed.
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what this limits: any lateralisation claim: A CRANIOTOMY WAS MADE ONLY OVER THE RIGHT AC
limitations[0]the tagger's words
limitationsIsoflurane was used as a model of the sleeping brain, and whether the state effects are anaesthetic or sleep-related is unresolved.
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what this limits: the sleep interpretation of the state effects: REPLICATION OF OUR FINDINGS DURING NATURAL SLEEP WILL CLARIFY WHETHER CHANGES IN HEARING SENSITIVITY RESULT FROM USING ISOFLURANE OR ARE ASSOCIATED WITH SLEEP PROCESSES
limitations[1]the tagger's words
limitationsThe comparison of natural sleep against awake and anaesthetized states is left to future work.
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what this limits: the same scope as limitations[1]: THE IMPACT OF SLEEP COMPARED TO ANESTHETIZED AND AWAKE STATES SHOULD BE STUDIED IN THE FUTURE
limitations[2]the tagger's words
limitationsThe map of this cortex has been revised repeatedly, and the paper attributes the revisions to advances in technique rather than to new anatomy.
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what this limits: any area label in the mouse auditory cortex literature: the paper states the tonotopic organisation of the belt area has been significantly updated as neuroimaging and recording advanced
limitations[3]1.0the tagger's words
limitationsAn area in the classical map is reported as dissolvable into parts of two others, on the evidence of one technique.
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what this limits: the UF area as a unit in the mouse auditory map: electrophysiological measurements are reported as suggesting it should be considered subparts of AAF and AI
limitations[4]1.0the tagger's words
limitationsThe tonotopic map rests on a per-frequency threshold identified by the experimenters.
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what this limits: the tonotopic maps in this paper: for each frequency a threshold level was identified and used to determine the map
limitations[5]1.0the tagger's words
limitationsThe paper's own account of why the map keeps changing is an account of instruments, not of findings - decades of technological advancement producing significant updates to the organisation. On that account the current map is the current technique's map, and the paper is adding a fourth.
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limitations[6]0.9the tagger's words
controlsa deliberately LARGE craniotomy, 3 to 4 mm across, over the auditory cortical regions
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eliminates: that the map is shaped by the size of the recording window rather than by the cortex - a small window would truncate the functional organisation being measured · outcome (stated by the tagger, no quotation): held
controls[0]0.95the tagger's words
controlsresponsive pixels were identified first and used to exclude the unresponsive ones from analysis
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eliminates: that unresponsive pixels dilute the signal and flatten the functional map · outcome (stated by the tagger, no quotation): held
controls[1]0.95the tagger's words
conceptstonotopic organization
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concepts[0]the paper's words
conceptsUF
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also in: UF
concepts[1]the paper's words
conceptsAI
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also in: AI
concepts[2]the paper's words
conceptsauditory cortex
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concepts[3]0.95the paper's words
sensesAI (primary auditory cortex) - AS ONE AREA in the classical map, versus as an area containing TWO high-frequency regions, which is what optical calcium imaging reported
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senses[0]1.0the tagger's words
sensesAI (primary auditory cortex) - AND AS SPLIT DIFFERENTLY AGAIN by a third technique: flavoprotein autofluorescence with anatomy makes the dorsal high-frequency part a discrete region
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senses[1]1.0the tagger's words
sensesAI (primary auditory cortex) - AND AS PART OF A WHOLLY NEW LAYOUT: widefield calcium imaging in awake mice is reported as introducing a new layout for auditory cortex
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senses[2]1.0the tagger's words
sensesrevision of the map - attributed by the paper to TECHNIQUE rather than to anatomy: 'technological advancements in neuroimaging and recording have led to significant updates in the tonotopic organization of the belt area'
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senses[3]0.95the tagger's words
claim_relationscontradicts → Issa et al. (2017), which reported no difference between upward and downward FM sweep maps
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claim_relations[0]the tagger's words
claim_relationsextends → Issa et al. (2017), whose imaging window was too small to resolve map borders
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claim_relations[1]the tagger's words
claim_relationsextends → Romero et al. (2020), whose VPAF is placed differently
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claim_relations[2]the tagger's words
claim_relationsbuilds-on → Issa et al. (2014) landmark-registration procedure
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claim_relations[3]the tagger's words
concept_edgesUF —part-of→ AI
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differs in: WHICH TECHNIQUE IS ASKED. 'ELECTROPHYSIOLOGICAL MEASUREMENTS suggest the UF area should be considered as SUBPARTS of the AAF and AI' - so on that instrument UF is not an area at all but pieces of two others. The record already carries THREE readings of AI as senses, one per technique: optical calcium imaging splits it into two high-frequency regions, flavoprotein autofluorescence makes the dorsal part discrete, widefield imaging introduces a new layout entirely · strength of the relation: 0.7 · note: AN AREA THAT DISSOLVES INTO PARTS OF TWO OTHERS DEPENDING ON THE METHOD. The boundary is not a fact the techniques disagree about - each technique constitutes a different partition, and all of them are current. Audit 2026-09-28: DOUBTFUL - read against its full span and kept, but measurements 'suggest' UF should be considered subparts of AAF and AI; hedged.
also in: UF · AI
concept_edges[0]0.85the tagger's words
content_tagsKruskal-Wallis test
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content_tags.statistics[0]the tagger's words
content_tagschi-squared goodness-of-fit test
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content_tags.statistics[1]the tagger's words
content_tagsbootstrap
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also in: bootstrap
content_tags.statistics[2]the tagger's words
content_tagsWilcoxon rank-sum test
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content_tags.statistics[3]the tagger's words
content_tagsp-value
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also in: p-value
content_tags.statistics[4]the tagger's words
content_tagsstandard error of the mean
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content_tags.statistics[5]the tagger's words
content_tagsregression
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also in: regression
content_tags.statistics[6]the tagger's words
content_tagscoefficient of determination
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content_tags.statistics[7]the tagger's words
content_tagsexplained variance
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content_tags.statistics[8]the tagger's words
content_tagssingular value decomposition
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content_tags.statistics[9]the tagger's words
content_tagsstandard deviation
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content_tags.statistics[10]the tagger's words
content_tagsThe newly identified auditory cortex regions responded mainly to higher frequencies and lay in dorsal and caudal auditory cortex.
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content_tags.findings[0]the tagger's words
content_tagsApart from a few sparse pixels, tonotopic maps were similar over time and between animals.
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content_tags.findings[1]the tagger's words
content_tagsHigh-frequency responses in AI-H and UF were larger in awake than in anesthetized mice.
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content_tags.findings[2]the tagger's words
content_tagsUnder anesthesia the cortical area encoding slow FM sweep rates expanded and the area encoding fast sweep rates shrank across AAF, DA and UF.
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content_tags.findings[3]the tagger's words
content_tagsContrary to an earlier report of no difference, upward and downward FM sweep maps do differ: the slow-rate centre in AI sits about 350 micrometres more rostral for downward sweeps.
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content_tags.findings[4]the tagger's words
content_tagsUltrasonic vocalizations drove stronger responses than pure tones specifically in the regions tuned to fast FM sweep rates (AII, DP, PAF, VPAF and a zone between UF and AAF), and not in the high-frequency tonotopic regions.
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content_tags.findings[5]the tagger's words
content_tagsBest frequency changes with distance along a tonotopic gradient as a sigmoid: middle frequencies change steeply over a short stretch of cortex while low and high frequencies occupy longer stretches.
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content_tags.findings[6]the tagger's words
content_tagsEVIDENCE CORRECTED (RE-GROUNDED - THE POINTER WAS WRONG, NOT THE CLAIM) · Tonotopic maps were almost identical for averaging windows from 100 ms to 1000 ms after tone onset.
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EVIDENCE CORRECTED: RE-GROUNDED - THE POINTER WAS WRONG, NOT THE CLAIM. The old evidence was verbatim, anchored and correctly cut, and it contained NONE of this claim's numbers (100, 1000). The paper states the claim in one sentence elsewhere and the tagger did not use it. the project, 2026-08-23: 'WE HAVE SPENT THE DAY VERIFYING THAT QUOTES ARE REAL AND NONE OF IT ASKS WHETHER THEY ARE RELEVANT.' Confirmed by reading the claim, the old span and the candidate together; the candidate carries every number AND the claim's own content words.
content_tags.findings[7]the tagger's words
content_tagsA window larger than 2 mm in each axis is needed to include every region of the tonotopic organisation.
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content_tags.findings[8]the tagger's words
content_tagscore auditory area
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content_tags.definitions[0]the tagger's words
content_tagsbelt auditory region
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content_tags.definitions[1]the tagger's words
content_tagstonotopic organization
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content_tags.definitions[2]the tagger's words
content_tagsfrequency sweep rate
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content_tags.definitions[3]the tagger's words
content_tagsV/T ratio
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also in: V/T ratio
content_tags.definitions[4]the tagger's words
content_tagsresponsive pixel
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content_tags.definitions[5]the tagger's words
content_tagstone frequency and sound pressure level
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content_tags.variables[1]the tagger's words
content_tagsbest frequency
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content_tags.variables[3]the tagger's words
content_tagspixel responsiveness
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content_tags.variables[4]the tagger's words
content_tagsbody temperature
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content_tags.variables[5]the tagger's words
content_tagssound pressure level
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content_tags.variables[6]the tagger's words
content_tagsfrequency coverage
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content_tags.variables[7]the tagger's words
content_tagssweep direction
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content_tags.variables[9]the tagger's words
content_tagssweep duration
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content_tags.variables[10]the tagger's words
content_tags12 and 6 — Number of mice recorded awake and anesthetized respectively
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content_tags.quantities[0]the tagger's words
content_tagsR2 > 0.93 and 0.90 — Adjusted R-squared of the linear fit to the inverse-sigmoid frequency-distance relation, awake and anesthetized
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content_tags.quantities[1]the tagger's words
content_tagsR2 > 0.95 and 0.87 — Fit quality of the inverse-sigmoid function for the FM sweep-rate/distance relation, awake and anesthetized
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content_tags.quantities[2]the tagger's words
content_tags1.36 and 1.51 — Median vocalization-to-tone response ratio in the USV-favouring pixels, awake and anesthetized
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content_tags.quantities[3]the tagger's words
content_tags2 mm and 1.9 mm — Extent of the auditory cortex tonotopic map along the rostral-caudal and dorsal-ventral axes
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content_tags.quantities[4]the tagger's words
content_tagsAI-L p = 0.0054, AI-H p = 0.0171, AAF-L p = 0.0181, UF p = 0.0216, DP p = 0.0165 — Median p-values for the five regions whose best frequency differed between awake and anesthetized maps
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about this quote: THE QUOTE CONTINUES A SENTENCE THAT THIS COPY CUTS IN TWO - a bioRxiv watermark stands between it and the words it follows, which read 'the high-frequency regions of AI, UF,'.
content_tags.quantities[5]the tagger's words
content_tags350 µm — Rostral shift of the AI slow-sweep-rate landmark in the downward compared with the upward FM map
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content_tags.quantities[6]the tagger's words
content_tags25 times — Repetitions of each frequency-SPL pair per imaging session
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content_tags.quantities[7]the tagger's words
content_tags33.3ms (30 Hz) — Temporal resolution / frame rate of the wide-field calcium imaging
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content_tags.quantities[8]the tagger's words

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