Architecture and organization of mouse posterior parietal cortex relative to extrastriate areas Hovde Gianatti Witter Whitlock

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 Architecture and organization of mouse posterior parietal cortex relative to extrastriate areas 2018 bioRxiv 10.1101/361832 hovde2018-architecture-and-organization-of-m hovde2018-architecture-and-organization-of-m.txt, the project's staged copy 7757a383734ad69ab87ec9b2f2827422ab6ac1e6 open-access neuroscience

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 0 spans located by searching the stored text for the quote's exact words, allowing any whitespace between them Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. observation Quotation withheld because this record is not cleared for redistribution. A WIDELY USED REGION NAME IS REPORTED TO HAVE NO COHERENT DEFINITION, AND THE COST IS NAMED: despite the popularity of the animal for studying the region there is LITTLE CONSENSUS ON A COHERENT ANATOMICAL DEFINITION, WHICH IS PROBLEMATIC BECAUSE IT COMPLICATES THE INTERPRETATION OF THE WEALTH OF NEW DATA. The missing definition is treated as a defect in the literature's ability to read its own results Quotation withheld because this record is not cleared for redistribution. THE PARTS THAT ARE ILL-DEFINED ARE ENUMERATED RATHER THAN GESTURED AT: what remains ILL-DEFINED is named as the BOUNDARIES which distinguish it from neighbouring areas, its LAMINAR ORGANIZATION and its CHEMOARCHITECTURAL PROFILE - three specific things, each of which a definition would have to fix Quotation withheld because this record is not cleared for redistribution. IN THE ABSENCE OF A DEFINITION, PRACTICE SUBSTITUTES TWO PROXIES, AND BOTH ARE NAMED: targeting has relied EITHER on functional mapping of the areas around the region OR on stereotactic coordinates followed by POST-HOC HISTOLOGICAL COMPARISON to one of SEVERAL REFERENCE ATLASES. Where the definition is missing, the coordinates and the atlas stand in for it Quotation withheld because this record is not cleared for redistribution. AND THE PROXIES ARE CONSISTENT WITHIN A STUDY AND INCOMPARABLE ACROSS STUDIES, FOR A STATED REASON: these conventions MAY YIELD CONSISTENT RECORDING LOCATIONS WITHIN A STUDY, BUT HAMPER THE COMPARISON of the region ACROSS STUDIES, SINCE THEY ARE BASED ON DIFFERENT LABELING METHODOLOGIES AND UNRELATED NOMENCLATURES. Reliability inside a lab and incommensurability between labs, traced to the naming systems themselves Quotation withheld because this record is not cleared for redistribution. THE CONTRIBUTION IS EXPLICITLY A RECONCILIATION OF NOMENCLATURES, NOT A NEW FINDING: the paper states that it RECONCILED WIDELY USED BUT DISPARATE NOMENCLATURES referring to the region VERSUS the areas around it - two naming systems, each with its own literature, mapped onto one another Quotation withheld because this record is not cleared for redistribution. THE NEW DEFINITION IS ASSEMBLED FROM FOUR INDEPENDENT KINDS OF EVIDENCE, AND THE LIST IS THE DEFINITION: the combined approaches are said to provide A COHERENT DEFINITION that INCORPORATES laminar architecture, extrastriate projections, thalamic connections and cortico-cortical connections. Nothing here is a criterion on its own; the definition is the conjunction Quotation withheld because this record is not cleared for redistribution. THE DEFINING CRITERIA ARE STAINS - THE DEFINITION IS OPERATIONALISED BY REAGENTS: the region is delineated using CYTOARCHITECTURAL AND LAMINAR CRITERIA obtained from Nissl-, parvalbumin- and type-2 muscarinic acetylcholine receptor-immunostained sections. What the term picks out is fixed by what three stains show Quotation withheld because this record is not cleared for redistribution. AND ONE STATED AIM IS THAT THE CRITERIA BE AVAILABLE TO EVERYONE: the study aims to provide A RESOURCE for identifying the region with anatomical criteria THAT ARE EVIDENT USING UBIQUITOUS STAINING METHODS, SUCH AS A NISSL STAIN. A definition is chosen partly for the commonness of the equipment needed to apply it Quotation withheld because this record is not cleared for redistribution. WHEN THE ANATOMY OFFERS NO LANDMARKS, FUNCTION IS BORROWED TO SERVE AS ONE: since the dorsal cortical surface in rodents LACKS GROSS ANATOMICAL LANDMARKS, the pattern of responses in the surrounding areas has provided INCREASINGLY-USED FUNCTIONAL LANDMARKS for locating higher visual and associative regions. A location is fixed by what a region DOES because there is nothing to see about this quote: The span contains 'increasinglyused' for 'increasingly-used' - a LOST-HYPHEN FUSION, damage shape 10. A line-break hyphen was dropped by the extraction and the halves fused. The quote is an exact substring and anchors correctly; the damage is in what a reader of the tag sees. None of the four shapes an internal tool looks for covers this, so it is annotated by hand. Quotation withheld because this record is not cleared for redistribution. AND THE BORROWED METHOD CARRIES ITS OWN PRECONDITION, WHICH IS STATED: functional mapping of that kind is NOT FEASIBLE IN THE ABSENCE OF a broadly expressed calcium indicator or intrinsic optical imaging - so the substitute for the missing definition is itself unavailable to laboratories without those tools Quotation withheld because this record is not cleared for redistribution. THE TWO NAMING SYSTEMS ARE FOUND TO OVERLAP PARTIALLY, NOT CLEANLY, AND THE PATTERN IS REPORTED PIECE BY PIECE: the anterior pole of the region DOES NOT OVERLAP with any of the neighbouring areas, the posterior sectors OVERLAP PARTLY with two of them, and its most lateral and posterior extent LARGELY OVERLAPPED with a third. Neither vocabulary's units nest inside the other's Quotation withheld because this record is not cleared for redistribution. A NAME IS SHOWN TO ASSERT SOMETHING ITS OWN EVIDENCE COMPLICATES: these areas ARE REFERRED TO AS EXTRASTRIATE, IMPLYING A PRIMACY OF VISUAL PROCESSING - THOUGH they OVERLAP CONSIDERABLY with a region the paper characterises as cognitive. The label's morphology makes a claim about what the territory is for, and the overlap is the counter-evidence about this quote: TRIMMED, not re-sliced: the sentence continues "...navigational and movement-related functions that can be expressed independently of visual input", which is the sharper half of the point - but the bioRxiv WATERMARK is spliced into the citation list mid-sentence ("cognitive (Harvey et al., 2012; 14 bioRxiv preprint ... doi: a ; Morcos & Harvey, 2016"), so no contiguous span reaches it. The tag claims only what the quote carries. Quotation withheld because this record is not cleared for redistribution. what this limits: any functional claim about integration in mouse PPC: IT REMAINS TO BE MAPPED MORE FULLY WHERE THE INPUTS FROM ASSOCIATIVE, MOTOR, AND SENSORY AREAS ARE INTEGRATED SYNAPTICALLY WITHIN PPC, AND WHETHER GRADED TOPOGRAPHIES EXIST FOR DIFFERENT SENSORY MODALITIES Quotation withheld because this record is not cleared for redistribution. what this limits: the reference frame the mapping used: THE PRESENT STUDY FOCUSED ON CHARACTERIZING PPC RELATIVE TO EXTRASTRIATE PROJECTIONS, and comparable mapping against somatosensory, auditory or motor inputs was not done Quotation withheld because this record is not cleared for redistribution. what this limits: the M2-receptor labelling in BDA-injected tissue: WE NOTED CROSS-REACTIVITY BETWEEN ANTIBODIES AGAINST M2-RECEPTORS AND BDA LABELING, controlled by comparison with tissue WITHOUT BDA INJECTIONS Quotation withheld because this record is not cleared for redistribution. what this limits: any comparison of PPC results across laboratories: the authors state that existing strategies may yield consistent recording locations within a study but hamper comparison across studies, because they rest on different labeling methodologies and unrelated nomenclatures Quotation withheld because this record is not cleared for redistribution. what this limits: any claim about the extent of mouse PPC: the boundaries distinguishing it from neighbouring areas, its laminar organization and its chemoarchitectural profile are said to remain ill-defined Quotation withheld because this record is not cleared for redistribution. what this limits: any use of mouse PPC as a defined region before 2018: the authors state that the laminar, cytoarchitectonic and chemoarchitectonic criteria they describe have not, to their knowledge, been established previously Quotation withheld because this record is not cleared for redistribution. eliminates: that the labelling attributed to M2 receptors is the tracer showing through - a known cross-reactivity, noticed and then bounded rather than ignored · outcome (stated by the tagger, no quotation): held Quotation withheld because this record is not cleared for redistribution. mouse PPC Quotation withheld because this record is not cleared for redistribution. nomenclatures Quotation withheld because this record is not cleared for redistribution. cytoarchitectural and laminar criteria Quotation withheld because this record is not cleared for redistribution. chemoarchitectural profile Quotation withheld because this record is not cleared for redistribution. labeling methodologies Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. mouse PPC cytoarchitectural and laminar criteria strength of the relation: 1 Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. coherent anatomical definition Quotation withheld because this record is not cleared for redistribution. flat maps about this quote: QUOTE RECOVERED FROM THE PAPER'S OWN CHARACTERS. The recorded quote was LETTERS-ONLY IDENTICAL to this stretch of the staged text but not an exact substring of it - spaces, hyphens, ligature glyphs or mathematical operators differed, so it could never anchor. This is the file's own text between the same two letters: not produced by a rule about the damage, copied out of the file. Round-trip checked (the recovered span is letters-only equal to the original) and length-checked. The text as originally recorded is kept in evidence as extracted. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution. Quotation withheld because this record is not cleared for redistribution.

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