Re-epithelialization and immune cell behaviour in an ex vivo human skin model

Re-epithelialization and immune cell behaviour in an ex vivo human skin model Ana Rakita Nenad Nikolić Michael Mildner Johannes Matiasek Adelheid Elbe‐Bürger

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Re-epithelialization and immune cell behaviour in an ex vivo human skin model 2020 Scientific Reports 10.1038/s41598-019-56847-4 rakita2020-re-epithelialization-and-immune-ce rakita2020-re-epithelialization-and-immune-ce.txt, the project's staged copy 9ece70bbab6832eac5ef22d1215f58ce2fb3e13f cc-by medicine

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0 spans located by searching the stored text for the quote's exact words, allowing any whitespace between them re-epithelialization and immune cell behaviour in an ex vivo human skin wound made by suction Quotation withheld because this record is not cleared for redistribution. whether a human ex vivo wound can be made without disrupting the dermal compartment Quotation withheld because this record is not cleared for redistribution. a suction-blister wound model in which the basement membrane survives and the wound bed starts free of keratinocytes Quotation withheld because this record is not cleared for redistribution. method Quotation withheld because this record is not cleared for redistribution. closure of an epidermal wound by keratinocyte migration and proliferation from the wound edges Quotation withheld because this record is not cleared for redistribution. experiment experiment Quotation withheld because this record is not cleared for redistribution. mechanisms EACH SEPARATION METHOD DAMAGES A DIFFERENT THING, WHICH IS WHY NONE IS GENERAL: heat harms epidermis and dermis, chemicals disturb the electrolyte equilibrium, enzymes kill components - and mechanical force is chosen because it changes nothing chemically Quotation withheld because this record is not cleared for redistribution. mechanisms THE MODEL'S WHOLE POINT IS WHAT IT LEAVES INTACT: the basement membrane survives blister roof removal and no keratinocytes remain in the wound bed, so closure afterwards must come from the edges Quotation withheld because this record is not cleared for redistribution. mechanisms EX VIVO IS SLOWER THAN IN VIVO AND THE PAPER SAYS WHY: culture lacks factors that arriving cells from the circulation would supply, and the dermal cells have been removed Quotation withheld because this record is not cleared for redistribution. EVERY EXPERIMENT SHOWN USES ABDOMINAL SKIN ONLY, stated by the authors in their own discussion Quotation withheld because this record is not cleared for redistribution. Re-epithelialization in the model is slower than in vivo, which the paper attributes to missing culture factors and to the removal of dermal cells Quotation withheld because this record is not cleared for redistribution. The model produces a differentiation pattern NOT seen in normal skin - DSG1 and CDSN in lower epidermal layers, read as premature cornification Quotation withheld because this record is not cleared for redistribution. No separation method is appropriate for all purposes, on the paper's own account - so the model is offered as one technique among several rather than as a replacement Quotation withheld because this record is not cleared for redistribution. The literature is described as large and yet lacking a standardised ex vivo model that leaves the dermal compartment undisrupted about this quote: the quote carries SMALL-CAPS RENDERED AS LOWERCASE: the paper's “Nonetheless” opens a sentence and is staged as “nonetheless”. Verbatim against the staged text and harmless to the reading, but it is why the sentence looks like a comma splice. Confidence 0.85 under an earlier note of ours. Quotation withheld because this record is not cleared for redistribution. the same suction applied to ex vivo thigh and arm skin, to show the method does not depend on the body site eliminates: that the re-epithelialization result is a property of abdominal skin, which is what was available - the paper says thigh and arm skin gave the same result and does not show it · outcome: absent-declared-unreported · would have ruled out, had it been run: the objection that the model works only on abdominal skin Quotation withheld because this record is not cleared for redistribution. negative pressure negative pressure Quotation withheld because this record is not cleared for redistribution. basement membrane basement membrane Quotation withheld because this record is not cleared for redistribution. blister roof blister roof Quotation withheld because this record is not cleared for redistribution. keratinocytes keratinocytes Quotation withheld because this record is not cleared for redistribution. re-epithelialization re-epithelialization Quotation withheld because this record is not cleared for redistribution. animal model animal model Quotation withheld because this record is not cleared for redistribution. separation technique separation technique Quotation withheld because this record is not cleared for redistribution. suction suction Quotation withheld because this record is not cleared for redistribution. epidermis epidermis Quotation withheld because this record is not cleared for redistribution. dermis dermis Quotation withheld because this record is not cleared for redistribution. abdominal skin abdominal skin Quotation withheld because this record is not cleared for redistribution. 3 R principle 3 R principle Quotation withheld because this record is not cleared for redistribution. cornification cornification Quotation withheld because this record is not cleared for redistribution. wound re-epithelialization wound re-epithelialization Quotation withheld because this record is not cleared for redistribution. terminal differentiation program terminal differentiation program Quotation withheld because this record is not cleared for redistribution. Hemidesmosomes Hemidesmosomes Quotation withheld because this record is not cleared for redistribution. desmosomes desmosomes Quotation withheld because this record is not cleared for redistribution. epithelial wound regeneration epithelial wound regeneration Quotation withheld because this record is not cleared for redistribution. dermal efflux dermal efflux Quotation withheld because this record is not cleared for redistribution. suction blister fluid suction blister fluid Quotation withheld because this record is not cleared for redistribution. MMP-9 MMP-9 Quotation withheld because this record is not cleared for redistribution. detaching basal keratinocytes detaching basal keratinocytes Quotation withheld because this record is not cleared for redistribution. wound closure wound closure Quotation withheld because this record is not cleared for redistribution. VEGF release VEGF release Quotation withheld because this record is not cleared for redistribution. angiogenesis angiogenesis Quotation withheld because this record is not cleared for redistribution. removal of dermal cells removal of dermal cells Quotation withheld because this record is not cleared for redistribution. slight delay of re-epithelialization slight delay of re-epithelialization Quotation withheld because this record is not cleared for redistribution. Topical negative pressure Topical negative pressure Quotation withheld because this record is not cleared for redistribution. ideal wound healing environment ideal wound healing environment Quotation withheld because this record is not cleared for redistribution. separation technique - THE PAPER TREATS THE CHOICE AS QUESTION-DEPENDENT RATHER THAN AS A RANKING: heat, chemicals, enzymes and mechanical force each damage something different, and which damage is acceptable depends on what is being asked Quotation withheld because this record is not cleared for redistribution. Re-epithelialization - epidermal regeneration completed by keratinocyte migration and differentiation Quotation withheld because this record is not cleared for redistribution. wound re-epithelialization - epidermal regeneration in which keratinocytes migrate and differentiate Quotation withheld because this record is not cleared for redistribution. terminal differentiation program - the differentiation transition in which basal K14/K5 are substituted by K1/K10 Quotation withheld because this record is not cleared for redistribution. Hemidesmosomes - basement-membrane attachments that loosen to permit keratinocyte migration Quotation withheld because this record is not cleared for redistribution. desmosomes - cell-cell attachments that loosen to permit keratinocyte migration Quotation withheld because this record is not cleared for redistribution. epithelial wound regeneration - the main regenerative features the ex vivo model is said to recapitulate Quotation withheld because this record is not cleared for redistribution. dermal efflux - movement inferred from dermal population decreases and their appearance in blister roof/fluid Quotation withheld because this record is not cleared for redistribution. suction blister fluid - one compartment in which populations absent from normal epidermis were detected Quotation withheld because this record is not cleared for redistribution. MMP-9 - wound-edge factor proposed to detach basal keratinocytes and promote closure Quotation withheld because this record is not cleared for redistribution. detaching basal keratinocytes - MMP-9-mediated release of basal keratinocytes from the basement membrane Quotation withheld because this record is not cleared for redistribution. wound closure - the repair outcome promoted by MMP-9-linked keratinocyte detachment and migration Quotation withheld because this record is not cleared for redistribution. VEGF release - release reported to be increased by MMP-9 Quotation withheld because this record is not cleared for redistribution. angiogenesis - vascular growth promoted by MMP-9 and dependent on endothelial MMP-9 expression Quotation withheld because this record is not cleared for redistribution. removal of dermal cells - loss introduced by suction blistering that might slow re-epithelialization Quotation withheld because this record is not cleared for redistribution. slight delay of re-epithelialization - the modest re-epithelialization slowdown the paper says may follow cell removal or absent incoming cells Quotation withheld because this record is not cleared for redistribution. Topical negative pressure - clinical negative-pressure treatment for difficult wounds Quotation withheld because this record is not cleared for redistribution. ideal wound healing environment - the local state attributed to increased flow, reduced bacterial colonization, and exudate removal Quotation withheld because this record is not cleared for redistribution. MMP-9 —causes→ VEGF release MMP-9 VEGF release the relation itself is separately evidenced · strength of the relation: 0.9 · note: The review says MMP-9 'has been reported to increase' VEGF release; weight 0.9 preserves the attribution. Audit 2026-09-28: DOUBTFUL - read against its full span and kept, but attributed: the span says MMP-9 'has been reported' to increase VEGF release. Quotation withheld because this record is not cleared for redistribution. MMP-9 —causes→ angiogenesis MMP-9 angiogenesis the relation itself is separately evidenced · strength of the relation: 0.9 · note: The review reports promotion and a prerequisite relation rather than presenting a new intervention; weight 0.9 preserves that status. Audit 2026-09-28: DOUBTFUL - read against its full span and kept, but attributed: the span says MMP-9 'has been reported' to promote angiogenesis. Quotation withheld because this record is not cleared for redistribution. removal of dermal cells —causes→ slight delay of re-epithelialization removal of dermal cells slight delay of re-epithelialization the relation itself is separately evidenced · strength of the relation: 0.7 · note: The paper says removal and/or absent incoming cells 'might cause' the delay; weight 0.7 preserves both modality and alternative causation. Audit 2026-09-28: DOUBTFUL - read against its full span and kept, but the span says the removal MIGHT cause a slight delay; hedged. Quotation withheld because this record is not cleared for redistribution. Topical negative pressure —causes→ ideal wound healing environment Topical negative pressure ideal wound healing environment the relation itself is separately evidenced · strength of the relation: 0.9 · note: The paper summarizes the clinical description of benefit through three named mechanisms; weight 0.9 marks that this is reported background rather than the paper's experiment. Audit 2026-09-28: DOUBTFUL - read against its full span and kept, but attributed: the span says topical negative pressure 'is described as' beneficial. Quotation withheld because this record is not cleared for redistribution.

The full text of this article is not redistributable, so it is not included here. The evidence sentences are withheld with it; the tag layer and its grounding status remain. The article itself is at https://doi.org/10.1038/s41598-019-56847-4.