Driving fast-spiking cells induces gamma rhythm and controls sensory responses

Cardin, Carlén, Meletis, Knoblich, Zhang, Deisseroth, Tsai, Moore · Nature · 2009 · neuroscience · 10.1038/nature08002 · record Cardin2009-driving-fast-spiking-cells-induces-gamma-rhy
tag layer · index · concepts · authors · עברית
Where this record's text came from. faceted 2026-08 by reading the locally held text (xml); every evidence string verified as an exact substring of that text before merging. limitations is EMPTY: the paper nowhere states a limit on its own conclusions. The nearest candidate, 'Quotation withheld', is reported as a result of the preparation and is never framed as a constraint on the claims, so under rule 6 I did not convert it into a limitation. The single claim_relation is 'builds-on' because the paper positions itself as the first causal test of an existing hypothesis rather than as a repetition of a prior experiment; the hypothesis is named in the authors' own words even though its sources are cited only by numeral. method is 'electrophysiology' (in vivo extracellular single-unit, intracellular whole-cell and LFP recording combined with channelrhodopsin-2 stimulation) - the facet vocabulary has no separate optogenetics value. 2026-08-12, 2026-08-14 and 2026-08-15 schema-0.3 passes: every quote verified as an exact substring of the staged text and anchored at merge time; concept terms verified present; every edge endpoint an existing node.
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Other fields define these differently the same word, defined another way in another discipline
precision 10 fields · standard deviation 3 fields · cell type 2 fields

Tags on this paper

Every tag the record carries, each linking to every other paper in the corpus that carries it. A tag appears here whether or not its words occur in the article — the spans below are only the ones a sentence in this paper licenses. Of the 17 concept tags on this paper, 2 are grounded in a quoted sentence. All carry the tagger's gloss of what the term means in this paper, which is not something the article can license.
gamma oscillations fast-spiking regular spiking channelrhodopsin-2 optogenetic parvalbumin local field potential double dissociation resonant circuit property inhibitory postsynaptic potentials barrel cortex vibrissa phase Cre precision AMPA spike probability Rhythmic FS-generated IPSPs gate sensory transmission: inhibition blocks input at its peak and releases it during decay, temporally sharpening cortical responses Increased FS firing recruits a resonance in the surrounding cortical network whose preferred band is set by the circuit's synaptic and biophysical properties channelrhodopsin-2 (ChR2) cortical gamma oscillations (20–80 Hz) local field potential (LFP) double dissociation of cell-type-specific state induction cell type timing of the whisker deflection relative power precision of sensory-evoked spikes LFP power light intensity untransduced contralateral barrel cortex t-test paired t -test ANOVA Kruskal-Wallis test Kruskal–Wallis test with Dunn's post-test standard error of the mean mean ± s.e.m. standard deviation electrophysiology

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relation to other workclaim_relationshow this paper stands to another: agrees, contradicts, extends, qualifies
claim typeclaim_typethe shape of the claim: existence, mechanism, method, critique, prediction, null
concept edgeconcept_edgesan asserted relation between two concepts, with the sentence asserting it
conceptconceptsan idea the paper works with, quoted where the paper uses it
content tagcontent_tagsa finding, definition, quantity, statistic or variable named in the paper
controlcontrolsan experiment run to eliminate a rival explanation
limitationlimitationswhat the paper says its evidence cannot support
mechanismmechanismshow the reported effect is said to come about
methodmethodhow the paper produced its evidence
object of studyobject_of_studywhat the paper is about — the thing it investigates
phenomenonphenomenonthe effect or regularity under study
sensesensesa distinct use of a term, quoted where the paper fixes it
transfertransfersa method or concept carried in from another field

The paper

The full text is not reproduced here. This article is not cleared for redistribution, so the page carries the tag layer only. Its evidence sentences are withheld; the grounding status remains with each tag, and the article itself is at https://doi.org/10.1038/nature08002. The reading view exists for the papers whose licence permits it.

Every tag on this page

claim_relations 4 · claim_type 1 · concept_edges 7 · concepts 2 · content_tags 46 · controls 1 · limitations 5 · mechanisms 8 · method 1 · object_of_study 1 · phenomenon 1 · senses 6 · transfers 1

Relations on this page carry a quotation for the claim. Where a relation was separately evidenced — and so tested against the vocabulary for its type — the row says so; 1 of 7 relations here carries that mark. The rest are the tagger’s reading of the sentence quoted beside them.

facettagfieldconf.whose words
object_of_studyWhether driving one cortical cell type is enough to create a network rhythm, and what that rhythm then does to sensory transmission.
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object_of_studythe paper's words
claim_typemechanism
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claim_typethe paper's words
phenomenoncortical gamma oscillations
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phenomenonthe paper's words
methodelectrophysiology
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methodthe paper's words
mechanismsmechanisms
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mechanisms[0]the tagger's words
mechanismsmechanisms
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mechanisms[1]the tagger's words
mechanismsSynchronous inhibition from fast-spiking cells opens a narrow window in which excitation is effective, which is what synchronises the ensemble
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mechanisms[2]the tagger's words
mechanismsThe gamma band is a property of the circuit rather than of the drive: changing light intensity changes how much amplification occurs but not which frequencies are amplified
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mechanisms[3]the tagger's words
mechanismsStimulating at random frequencies still produces gamma, so the rhythm is emergent and does not have to be imposed
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mechanisms[4]the tagger's words
mechanismsThe induced rhythm still needs excitatory feedback - blocking glutamate receptors abolishes it even while the interneurons keep firing
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mechanisms[5]the tagger's words
mechanismsThe functional consequence: inhibition blocks input at its peak and permits it after decay, which sharpens the timing of sensory responses
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mechanisms[6]the tagger's words
mechanismsCell-type targeting works through Cre-dependent expression, so the light acts only on neurons that already carry the driver
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mechanisms[7]the tagger's words
limitationsThe effect was not obtained in layer 5, so the result is confined to the layers where activation was observed
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what this limits: the laminar scope of the effect: WE DID NOT OBSERVE LIGHT ACTIVATION OF LAYER 5 FS CELLS (n=12 SITES IN 7 ANIMALS)
limitations[0]the tagger's words
limitationsLabelling efficiency falls off with distance from the injection, so the manipulated population is not uniform across the field
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what this limits: what 'the PV population' means at any distance from the injection: THE LABELLING EFFICIENCY OF AAV DIO ChR2-mCHERRY VARIED OVER DISTANCE FROM THE INJECTION SITE; CLOSE TO THE CENTRE OF THE INJECTION, >97% OF THE PV+ INTERNEURONS EXPRESSED ChR2-mCHERRY
limitations[1]the tagger's words
limitationsThe excitatory comparison covers at most half the target population, against near-complete coverage on the inhibitory side
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about this quote: FLATTENED EXPONENT IN THIS QUOTE - THE NUMBER IS WRONG BY ORDERS OF MAGNITUDE AS IT STANDS. The staged text reads '8.4×10 6', where the paper writes 10 to the power 6. The superscript was pushed onto the line by the PDF extractor. The quote is verbatim to the staged text and its anchor is sound; the STAGED TEXT is what is damaged. NOT repaired - repair means re-staging the body and re-anchoring the record. · what this limits: the excitatory arm of the FS-versus-RS comparison, which is covered less completely than the inhibitory arm: AT LEAST 50% OF THE aCamKII+ NEURONS IN LAYER 2/3 EXPRESSED ChR2-mCHERRY (913 OF 1,638 CELLS) · for which use evidence status note: FLATTENED EXPONENT IN THIS QUOTE - THE NUMBER IS WRONG BY ORDERS OF MAGNITUDE AS IT STANDS. The staged text reads '8.4×10 6', where the paper writes 10 to the power 6. The superscript was pushed onto the line by the PDF extractor. The quote is verbatim to the staged text and its anchor is sound; the STAGED TEXT is what is damaged. NOT repaired - repair means re-staging the body and re-anchoring the record.
limitations[2]the tagger's words
limitationsThe pyramidal-drive comparison at 40 Hz produced a small increase that did not reach significance, so the dissociation is asymmetric in strength
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what this limits: the frequency specificity of the dissociation: RS STIMULATION AT 8 Hz CAUSED A SIGNIFICANT INCREASE OF LFP POWER AT 8 Hz (P<0.001), WHEREAS RS STIMULATION AT 40 Hz CAUSED ONLY A SMALL, NONSIGNIFICANT INCREASE IN 40-Hz LFP POWER
limitations[3]the tagger's words
limitationsThe phase-gating experiment rests on twenty cells in three animals
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what this limits: the phase-gating result: n=20 CELLS IN 3 ANIMALS, responses to a single vibrissa deflection at one of five phases relative to a single gamma cycle
limitations[4]the tagger's words
transfersa microbial light-sensitive cation channel is imported as the tool for driving a specific mammalian cortical cell type in vivo
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transfers[0]the tagger's words
controlseach imaging channel collected in MULTI-TRACK mode rather than simultaneously
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eliminates: optical cross-talk between channels - bleed from one fluorophore into another channel would appear as co-labelling that is not there · outcome (stated by the tagger, no quotation): held
controls[0]0.9the tagger's words
conceptsgamma oscillations
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concepts[0]the paper's words
conceptsfast-spiking
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also in: fast-spiking
concepts[1]the paper's words
sensesthe causal claim - SUFFICIENCY: driving fast-spiking cells is enough to produce gamma, which is what the optogenetic activation shows
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senses[0]1.0the tagger's words
sensesthe causal claim - NECESSITY: naturally occurring gamma depends on the same cells, shown by shifting its phase with a single pulse
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senses[1]the tagger's words
sensesgamma - INDUCED by light: the rhythm created by driving interneurons, which is what most of the paper measures
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senses[2]1.0the tagger's words
sensesgamma - NATURALLY occurring: the spontaneous rhythm, a different object that the induced one is shown to connect with
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senses[3]1.0the tagger's words
sensesthe frequency band - as set by the DRIVE: the alternative in which spike probability determines which band is enhanced
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senses[4]1.0the tagger's words
sensesthe frequency band - as set by the CIRCUIT: resonance, which is what varying light intensity showed
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senses[5]1.0the tagger's words
claim_relationsbuilds-on → the fast-spiking-gamma hypothesis (prior computational models and recordings holding that gamma is generated by synchronous fast-spiking interneuron activity)
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claim_relations[0]the tagger's words
claim_relationssupports → the fast-spiking-gamma hypothesis derived from computational modelling
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claim_relations[1]the tagger's words
claim_relationssupports → the account of gamma as gating synaptic input rhythmically to synchronise cell assemblies
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claim_relations[2]the tagger's words
claim_relationsextends → prior in vivo recordings showing sensory-evoked gamma and phase-locked pyramidal firing
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claim_relations[3]the tagger's words
concept_edgesfast-spiking —causes→ gamma oscillations
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direction on the measure: increases · the relation itself is separately evidenced · strength of the relation: 1 · the relation stated: driving the interneurons AMPLIFIES gamma, selectively across a wide drive range
concept_edges[0]the tagger's words
concept_edgesregular spiking —distinct-from→ gamma oscillations
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strength of the relation: 0.9 Audit 2026-09-28: DOUBTFUL - read against its full span and kept, but pyramidal activation amplifies ONLY LOWER-frequency oscillations, a double dissociation; distinct-from gamma is loose.
concept_edges[1]the tagger's words
concept_edgesfast-spiking —causes→ inhibitory postsynaptic potentials
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strength of the relation: 1
concept_edges[2]the tagger's words
concept_edgesinhibitory postsynaptic potentials —causes→ precision
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strength of the relation: 1
concept_edges[3]the tagger's words
concept_edgesphase —causes→ precision
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strength of the relation: 0.9 Audit 2026-09-28: DOUBTFUL - read against its span and kept, but the span says precision was enhanced in a gamma-phase-dependent manner; 'phase causes precision' is loose.
also in: phase · precision
concept_edges[4]the tagger's words
concept_edgesAMPA —causes→ gamma oscillations
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strength of the relation: 0.9
concept_edges[5]the tagger's words
concept_edgesresonant circuit property —contrasted-with→ spike probability
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strength of the relation: 1
concept_edges[6]the tagger's words
content_tagst-test
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also in: t-test
content_tags.statistics[0]the tagger's words
content_tagsANOVA
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also in: ANOVA
content_tags.statistics[1]the tagger's words
content_tagsKruskal-Wallis test
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content_tags.statistics[2]the tagger's words
content_tagsstandard error of the mean
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content_tags.statistics[3]the tagger's words
content_tagsstandard deviation
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content_tags.statistics[4]the tagger's words
content_tagsOptogenetically driving fast-spiking interneurons across a wide frequency range amplified oscillations selectively in the gamma band.
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content_tags.findings[0]the tagger's words
content_tagsDriving FS cells at 20-80 Hz significantly amplified local field potential power at those frequencies.
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content_tags.findings[1]the tagger's words
content_tagsLFP power was selectively amplified in the gamma range whatever the light intensity or spike probability, though the drive affected the size of the enhancement.
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content_tags.findings[2]the tagger's words
content_tagsA single 1-ms light pulse to FS cells produced large fast IPSPs in nearby regular-spiking neurons, confirming direct monosynaptic inhibition.
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content_tags.findings[3]the tagger's words
content_tagsActivating FS cells suppressed whisker-evoked responses in neighbouring excitatory neurons.
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content_tags.findings[4]the tagger's words
content_tagsThe phase of the gamma cycle at which a whisker deflection arrived determined the amplitude, latency and temporal precision of the resulting cortical response.
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content_tags.findings[5]the tagger's words
content_tagsLight-induced gamma depended on fast excitatory synaptic transmission, since blocking AMPA and NMDA receptors abolished it even while FS cells still fired strongly.
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content_tags.findings[6]the tagger's words
content_tagsA brief FS activation reset the phase of spontaneously occurring gamma oscillations, linking naturally occurring gamma to FS activity.
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content_tags.findings[7]the tagger's words
content_tagsdriving fast-spiking interneurons amplified the local field potential selectively in the 20-80 Hz band
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content_tags.findings[8]the tagger's words
content_tagsdriving excitatory neurons at 8-24 Hz raised LFP power at those frequencies but not at higher ones
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content_tags.findings[9]the tagger's words
content_tagsthe amplified band did not move with light intensity or spike probability, so it is set by the circuit
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content_tags.findings[10]the tagger's words
content_tagssingle pulses shifted the phase of naturally occurring gamma, linking the induced rhythm to the spontaneous one
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content_tags.findings[11]the tagger's words
content_tagsthe position of a whisker deflection within the gamma cycle changed the amplitude, latency and precision of the response
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content_tags.findings[12]the tagger's words
content_tagsactivating the interneurons blocked whisker-evoked responses in neighbouring excitatory cells
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content_tags.findings[13]the tagger's words
content_tagschannelrhodopsin-2 (ChR2)
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content_tags.definitions[0]the tagger's words
content_tagscortical gamma oscillations (20–80 Hz)
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content_tags.definitions[1]the tagger's words
content_tagslocal field potential (LFP)
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content_tags.definitions[2]the tagger's words
content_tagsdouble dissociation of cell-type-specific state induction
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content_tags.definitions[3]the tagger's words
content_tagschannelrhodopsin-2
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content_tags.definitions[4]the tagger's words
content_tagsgamma oscillations
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content_tags.definitions[5]the tagger's words
content_tagslocal field potential
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content_tags.definitions[6]the tagger's words
content_tagscell type
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also in: cell type
content_tags.variables[1]the tagger's words
content_tagstiming of the whisker deflection
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content_tags.variables[3]the tagger's words
content_tagsrelative power
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content_tags.variables[4]the tagger's words
content_tagsspike probability
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content_tags.variables[5]the tagger's words
content_tagsprecision of sensory-evoked spikes
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content_tags.variables[6]the tagger's words
content_tagsLFP power
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also in: LFP power
content_tags.variables[11]the tagger's words
content_tagslight intensity
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content_tags.variables[12]the tagger's words
content_tagsuntransduced contralateral barrel cortex
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content_tags.variables[13]the tagger's words
content_tags96.7±1.0% — proportion of ChR2-mCherry-expressing neurons that were parvalbumin-positive, confirming targeting specificity
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content_tags.quantities[0]the tagger's words
content_tags−67.6±1.9 mV — mean reversal potential of the light-evoked IPSPs, consistent with a GABA-A chloride conductance
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content_tags.quantities[1]the tagger's words
content_tags2.7±1.0 mV — mean peak amplitude of the light-evoked IPSP measured near −55 to −60 mV
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content_tags.quantities[2]the tagger's words
content_tags0.5 to 0.75 ms — lead time by which light-evoked FS spikes preceded the onset of the postsynaptic IPSP
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content_tags.quantities[3]the tagger's words
content_tagsn =64 FS cells in 15 animals — number of fast-spiking single units recorded in PV–Cre mice (against 56 RS cells in 7 αCamKII–Cre mice)
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content_tags.quantities[4]the tagger's words
content_tags9.1 ± 0.08 ms — onset latency of layer 4 regular-spiking responses to whisker deflection
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content_tags.quantities[5]the tagger's words
content_tags20–80 Hz — the gamma band, the frequency range selectively amplified
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content_tags.quantities[6]the tagger's words
content_tags96.7±1.0% — specificity of the targeting - labelled cells that really were parvalbumin-positive
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content_tags.quantities[7]the tagger's words
content_tagsn =64 FS cells in 15 animals — the inhibitory recording sample
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content_tags.quantities[8]the tagger's words
content_tags0.5 to 0.75 ms — how far the interneuron spike preceded the inhibitory potential it caused
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content_tags.quantities[9]the tagger's words
content_tags−67.6±1.9 mV — the reversal potential identifying the current as GABA-A mediated
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content_tags.quantities[10]the tagger's words
content_tagsfive phases — the positions in the gamma cycle at which the whisker was deflected
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content_tags.quantities[11]the tagger's words

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