Behavior-dependent short-term assembly dynamics in the medial prefrontal cortex

Fujisawa, Amarasingham, Harrison, Buzsáki · Nature Neuroscience · 2008 · neuroscience · 10.1038/nn.2134 · record Fujisawa2008-behavior-dependent-short-term-assembly-dynam
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Where this record's text came from. faceted 2026-08 by reading the locally held text (html); every evidence string verified as an exact substring of that text before merging. The paper is really two contributions in one: the mechanism claim recorded here about behaviour-dependent spike transmission, and a substantial statistical-methods contribution (spike-jitter resampling with pointwise and global bands, plus a thinning procedure to dissociate firing rate from detectability) that a single 'method' facet cannot express. Prior work in this source is cited by superscript number without author names, so claim relations targets are described by reference number; the 'contradicts' relation is anchored to the paper's own 'In contrast' sentence, whose target is named in the immediately preceding sentence rather than within the quoted sentence itself. 2026-08-13, 2026-08-14 and 2026-08-15 schema-0.3 passes: every quote verified as an exact substring of the staged text and anchored at merge time; concept terms verified present; every edge endpoint an existing node.
2 quotations on this page contain a reference number where the paper printed a superscript. This journal style prints citations as raised numerals — parameters12,13 — and the extraction flattened them into the line, so the sentence reads parameters 12 , 13 . The digits are citation markers and not part of what the author wrote. The quotes keep them for the same reason they keep every other extraction fault: a quote is reproduced exactly or it proves nothing.
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Other fields define these differently the same word, defined another way in another discipline
working memory 4 fields · depression 3 fields · standard deviation 3 fields · confidence interval 2 fields · facilitation 2 fields · power 2 fields · and 1 more →

Tags on this paper

Every tag the record carries, each linking to every other paper in the corpus that carries it. A tag appears here whether or not its words occur in the article — the spans below are only the ones a sentence in this paper licenses. Of the 20 concept tags on this paper, 2 are grounded in a quoted sentence. All carry the tagger's gloss of what the term means in this paper, which is not something the article can license.
monosynaptic cross-correlogram jitter thinning power firing rates mPFC pyramidal interneuron short-term plasticity facilitation depression supralinear cell assemblies trajectory working memory goal representation central arm silicon probe dendritic spikes Short-term synaptic facilitation and depression modulate spike transmission, so sequentially discharging neurons form internally generated assemblies whose lifetimes are set by changing synaptic efficacies Supersynchronous presynaptic input triggers dendritic spikes in the target interneuron, producing supralinear summation of coincident inputs Neuronal reverberation: a receding assembly hands its representational content to a successor assembly, generating the ordered firing sequence functional synaptic efficacy spike train coincident-time offset spike surrogate data set position in the maze jittered data sets resampling / surrogate data methods Resampling methods permutation test permutation test (shuffling the first spike, second~ spike labels spike-jitter resampling for cross-correlogram significance kernel density estimation multiple-comparison correction (family-wise error rate) (global or family-wise) error rate (FWER) confidence interval (Exact) Clopper-Pearson confidence intervals t-test t -test standard deviation (mean ± s.d.) randomization by thinning (statistical power control) heuristic randomization argument based on thinning cross-correlation cross-correlograms Clopper-Pearson confidence intervals electrophysiology

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second readingalternativesan independent re-reading of a facet, recorded whether or not it agrees
relation to other workclaim_relationshow this paper stands to another: agrees, contradicts, extends, qualifies
claim typeclaim_typethe shape of the claim: existence, mechanism, method, critique, prediction, null
concept edgeconcept_edgesan asserted relation between two concepts, with the sentence asserting it
conceptconceptsan idea the paper works with, quoted where the paper uses it
content tagcontent_tagsa finding, definition, quantity, statistic or variable named in the paper
contributioncontributionwhat the paper claims to add
controlcontrolsan experiment run to eliminate a rival explanation
limitationlimitationswhat the paper says its evidence cannot support
mechanismmechanismshow the reported effect is said to come about
methodmethodhow the paper produced its evidence
object of studyobject_of_studywhat the paper is about — the thing it investigates
phenomenonphenomenonthe effect or regularity under study
sensesensesa distinct use of a term, quoted where the paper fixes it
transfertransfersa method or concept carried in from another field
unrecordable relationunhoused_relationsa relation the paper asserts that this vocabulary cannot express, with the reason

The paper

The full text is not reproduced here. This article is not cleared for redistribution, so the page carries the tag layer only. Its evidence sentences are withheld; the grounding status remains with each tag, and the article itself is at https://doi.org/10.1038/nn.2134. The reading view exists for the papers whose licence permits it.

Every tag on this page

alternatives 3 · claim_relations 7 · claim_type 1 · concept_edges 3 · concepts 2 · content_tags 71 · contribution 1 · controls 2 · limitations 10 · mechanisms 8 · method 1 · object_of_study 1 · phenomenon 1 · senses 5 · transfers 2 · unhoused_relations 1

Relations on this page carry a quotation for the claim. Where a relation was separately evidenced — and so tested against the vocabulary for its type — the row says so; none of 3 relations here carry that mark. The rest are the tagger’s reading of the sentence quoted beside them.

facettagfieldconf.whose words
object_of_studywhether short-term synaptic plasticity can be seen at work in an intact brain during behaviour
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object_of_studythe paper's words
contributionmonosynaptic efficacy varies with position and trajectory in a working memory task, beyond what co-varying firing rates can explain
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contributionthe paper's words
claim_typemechanism
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claim_typethe paper's words
phenomenonbehaviour-dependent short-term synaptic plasticity in prefrontal cortex
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phenomenonthe paper's words
methodelectrophysiology
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methodthe paper's words
mechanismsmechanisms
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status: the paper's own words: We hypothesize that
mechanisms[0]the tagger's words
mechanismsmechanisms
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mechanisms[1]the tagger's words
mechanismsmechanisms
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mechanisms[2]the tagger's words
mechanismsone presynaptic neuron's increased activity can reduce its control of an interneuron while another's increases it, on the same postsynaptic cell
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mechanisms[3]1.0the tagger's words
mechanismsthat opposite pattern on one cell argues for synaptic mechanisms rather than passive membrane properties
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mechanisms[4]1.0the tagger's words
mechanismscoincident discharge of two or more presynaptic neurons within 5 ms raises the target's firing probability supralinearly
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mechanisms[5]1.0the tagger's words
mechanismsdendritic spikes triggered by supersynchronous inputs are offered as a mechanism for that supralinearity
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mechanisms[6]0.8the tagger's words
mechanismssequences may reflect a receding assembly handing its content to the next, with lifetimes set by internal mechanisms including short-term plasticity
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mechanisms[7]0.7the tagger's words
limitationsOnly a small, possibly unrepresentative fraction of neurons and connections could be observed.
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what this limits: any population-level or connectivity claim: ONLY A SMALL PERCENTAGE OF NEURONS AND THEIR CONNECTIONS COULD BE MONITORED IN OUR STUDY
limitations[0]the tagger's words
limitationsSynaptic connections are inferred indirectly from extracellular cross-correlograms, not measured.
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what this limits: every reported synaptic connection: MONOSYNAPTIC INTERACTIONS CAN ONLY BE INDIRECTLY INFERRED FROM AN EXTRACELLULAR SIGNAL - inferred, not measured
limitations[1]the tagger's words
limitationsThe cross-correlation method is insensitive to weak pyramidal-to-pyramidal interactions.
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what this limits: the absence of weak pyramidal-to-pyramidal effects specifically: THE CROSS-CORRELATION METHOD MAY NOT RELIABLY DETECT AND ANALYZE WEAK EXCITATORY INTERACTIONS AMONG PRINCIPAL CELLS - a null here is not evidence of absence
limitations[2]the tagger's words
limitationsThe jitter window size was a judgement call, which qualifies literal reading of the significance values.
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what this limits: the significance values themselves: CHOOSING THE SIZE OF THE JITTER WINDOW WAS A MATTER OF JUDGMENT, AND THIS SHOULD BE KEPT IN MIND IN THE LITERAL INTERPRETATION OF SIGNIFICANCE
limitations[3]the tagger's words
limitationsThe position-dependence analysis is example-based, without corrections for multiple comparisons across cells.
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what this limits: the position-dependence analysis: OUR APPROACH HERE IS EXAMPLE-BASED, AND WE DID NOT EXPLICITLY COMPUTE MULTIPLE-COMPARISONS CONTROLS ACROSS CELLS
limitations[4]the tagger's words
limitationsThe population firing-rate estimates need confirmation by more direct methods.
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what this limits: the population firing-rate estimates only: THE PRESENT ESTIMATES SHOULD BE CONFIRMED BY FUTURE STUDIES USING MORE DIRECT METHODS
limitations[5]the tagger's words
limitationsonly a small percentage of neurons could be monitored, most are silent or sparsely active, and the population estimates are stated to need more direct methods
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what this limits: the same scope as limitations[0], with the added reason that most neurons are silent or sparsely active and so never enter the sample
limitations[6]1.0the tagger's words
limitationsthe cross-correlation method may not reliably detect weak excitatory interactions between principal cells, though it works for principal-to-interneuron connections
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what this limits: the same scope as limitations[2]
limitations[7]1.0the tagger's words
limitationsthe observed principal-to-interneuron ratio is lower than anatomy predicts, attributed to the recording method or to silent principal cells
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what this limits: any use of the cell-type composition as an anatomical estimate: the observed ratio IS LOWER THAN would be expected from anatomy, and the authors attribute the difference to the recording method
limitations[8]1.0the tagger's words
limitationsthe layer difference in trajectory selectivity is reported with the caveat that firing rate differences could influence it
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what this limits: the layer difference in trajectory selectivity: the authors report it with the caveat that firing-rate differences between layers could contribute to the effect
limitations[9]1.0the tagger's words
transfersconditional inference by surrogate construction, with the null chosen to preserve every timescale but the one under test
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transfers[0]1.0the tagger's words
transfersthinning - deleting observations at random until a nuisance variable is flat - imported as a power control
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transfers[1]0.8the tagger's words
controlsspike trains were jittered at a chosen timescale and the co-firing recomputed, so that only excess co-firing at the FINE timescale survives
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eliminates: that a co-firing pair is explained by shared slow input rather than by a monosynaptic connection - co-firing at broad timescales is common without any direct connection · outcome (stated by the tagger, no quotation): held
controls[0]0.95the tagger's words
controlsthe timing of the interaction was compared against what a passive membrane could produce
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eliminates: that the temporal structure is a passive electrical artefact - the membrane time constant or a changing input conductance in the postsynaptic cell · outcome (stated by the tagger, no quotation): held
controls[1]0.95the tagger's words
conceptssupralinear
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also in: supralinear
concepts[12]the paper's words
conceptsdendritic spikes
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concepts[19]the paper's words
unhoused_relationsvariation in firing rates → detectability
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RULED OUT BY THE AUTHORS AS AN EXPLANATION: because spikes are uniformly distributed across maze positions, the observed differences in monosynaptic activity are LESS LIKELY TO BE DUE TO firing-rate variation acting on detectability. A control argument, not a relation that holds - and no edge type can say a relation FAILS.
unhoused_relations[0]the tagger's words
sensesMONOSYNAPTIC here is an inference to the simpler explanation, not an observation - the named rival is shared input producing co-firing without any synapse, and the criterion that separates them is TIMESCALE
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senses[0]1.0the tagger's words
sensesthe surrogate is built so that its null contains everything EXCEPT the timescale of interest: jittering by ±5 ms preserves firing rates at broader timescales, so what survives cannot be explained by rate variation at tens of milliseconds
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senses[1]1.0the tagger's words
sensesfunctional synaptic efficacy is defined operationally as the magnitude of excess coincident spikes at short latency - it is a spike-count statistic, not a measured synaptic quantity
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senses[2]1.0the tagger's words
sensesthe independence assumption used by the standard shift-predictor approach is rejected here BY NAME, because broad-timescale effects alone can push short-latency co-occurrence above chance
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senses[3]1.0the tagger's words
sensesthe confound being controlled is DETECTABILITY rather than the effect: more spikes make a connection easier to detect, so a position-dependent rate would produce position-dependent detection with no change in efficacy
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senses[4]1.0the tagger's words
alternativesclaim_type: existence
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a second reading DIFFERING from the recorded value. Recorded: mechanism — note: a second reading, verbatim-verified, disagreeing with the value on the record. Not applied - a person decides.
alternatives.claim_typethe tagger's words
alternativesmethod: eight-shank silicon probe recording with jitter-surrogate cross-correlogram analysis and a spike-thinning control for detection power
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a second reading DIFFERING from the recorded value. Recorded: electrophysiology — note: a second reading, verbatim-verified, disagreeing with the value on the record. Not applied - a person decides.
alternatives.methodthe tagger's words
alternativesphenomenon: excess short-latency coincident spiking between a pyramidal cell and an interneuron, changing systematically across the maze
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a second reading DIFFERING from the recorded value. Recorded: behaviour-dependent short-term synaptic plasticity in prefrontal cortex — note: a second reading, verbatim-verified, disagreeing with the value on the record. Not applied - a person decides.
alternatives.phenomenonthe tagger's words
claim_relationsreplicates → previous studies reporting that short-timescale cross-correlations between neuron pairs vary with behaviour (refs 14-16, 33)
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claim_relations[0]the tagger's words
claim_relationscontradicts → those earlier studies' assumption that behaviourally varying short-latency cross-correlation peaks reflect pyramidal-pyramidal interactions
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claim_relations[1]the tagger's words
claim_relationsextends → prior in vitro observations that excitatory inputs from different sources onto the same interneuron can be depressing or potentiating (refs 2, 5)
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claim_relations[2]the tagger's words
claim_relationsreinterprets → earlier reports of behaviour-dependent short-term cross-correlations, assumed to reflect pyramidal-to-pyramidal interactions
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claim_relations[3]1.0the tagger's words
claim_relationsextends → in vitro evidence that pyramidal-to-interneuron synapses are plastic over tens to hundreds of milliseconds
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claim_relations[4]0.9the tagger's words
claim_relationsbuilds-on → jitter and surrogate methods for separating fine-timescale from broad-timescale synchrony
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claim_relations[5]1.0the tagger's words
claim_relationscritiques → the independence assumption underlying shift-predictor identification of monosynaptic connections
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differs in: THE AXIS IS THE INDEPENDENCE ASSUMPTION, STATED PRECISELY IN THE SPAN. 'Seeking more formal criteria for large scale analysis, a standard approach is Quotation withheld (that is, precisely, that the response of one neuron is conditionally exchangeable across trials, or shifts...).' The shift predictor is not wrong as arithmetic; it rests on an assumption the paper names in its formal form.
claim_relations[6]1.0the tagger's words
concept_edgesmonosynaptic —operationalised-as→ cross-correlogram
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strength of the relation: 1 · note: it can ONLY be inferred indirectly from an extracellular signal - the paper opens the section by saying so
concept_edges[0]1.0the tagger's words
concept_edgesfacilitation —contrasted-with→ depression
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strength of the relation: 1 · note: both found, and elsewhere BOTH ON ONE POSTSYNAPTIC CELL from different presynaptic partners, which is what rules out passive membrane properties
concept_edges[8]1.0the tagger's words
concept_edgescell assemblies —part-of→ mPFC
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strength of the relation: 0.8 · note: distributed across a large volume with no spatial clustering of similar tuning
also in: cell assemblies · mPFC
concept_edges[11]1.0the tagger's words
content_tagsresampling / surrogate data methods
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content_tags.statistics[0]the tagger's words
content_tagspermutation test
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content_tags.statistics[1]the tagger's words
content_tagsspike-jitter resampling for cross-correlogram significance
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content_tags.statistics[2]the tagger's words
content_tagskernel density estimation
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content_tags.statistics[3]the tagger's words
content_tagsmultiple-comparison correction (family-wise error rate)
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content_tags.statistics[4]the tagger's words
content_tagsconfidence interval
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content_tags.statistics[5]the tagger's words
content_tagst-test
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also in: t-test
content_tags.statistics[6]the tagger's words
content_tagsstandard deviation
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content_tags.statistics[7]the tagger's words
content_tagsrandomization by thinning (statistical power control)
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content_tags.statistics[8]the tagger's words
content_tagscross-correlation
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content_tags.statistics[9]the tagger's words
content_tagsjitter
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also in: jitter
content_tags.statistics[10]1.0the tagger's words
content_tagsClopper-Pearson confidence intervals
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content_tags.statistics[11]0.7the tagger's words
content_tagsOn the seconds timescale, transiently active mPFC neurons formed sequences that reliably distinguished the rat's left and right maze trajectories.
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content_tags.findings[0]the tagger's words
content_tagsOn the millisecond timescale, the efficacy of putative monosynaptic connections was modulated by task phase even after firing-rate co-variation was statistically accounted for.
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content_tags.findings[1]the tagger's words
content_tagsRoughly equal fractions of connected pairs showed firing-pattern-dependent synaptic depression and facilitation in the behaving animal.
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content_tags.findings[2]the tagger's words
content_tagsPresynaptic spikes coinciding within 5 ms drove the postsynaptic interneuron supralinearly, more than the sum of their separate effects.
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content_tags.findings[3]the tagger's words
content_tags16-18% of neurons also fired differentially in the central arm, where movement trajectories and head directions were apparently indistinguishable.
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content_tags.findings[4]the tagger's words
content_tagsPutative connection probability fell rapidly with the distance between somata.
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content_tags.findings[5]the tagger's words
content_tagsInformation in mPFC appears sparsely encoded by cell assemblies spread over a large volume.
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content_tags.findings[6]the tagger's words
content_tagsmonosynaptic efficacy varied with position in the maze and with left versus right trajectory rather than staying constant
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content_tags.findings[7]1.0the tagger's words
content_tags67 of 343 pairs with significant excitatory connections showed identifiable position dependence
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content_tags.findings[8]1.0the tagger's words
content_tags495 of 62,408 directed cell pairs, 0.79 percent, had significant short-latency narrow peaks or troughs
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content_tags.findings[9]1.0the tagger's words
content_tagsconnection probability fell rapidly with distance but connections were found up to 1,200 micrometres apart
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content_tags.findings[10]1.0the tagger's words
content_tagsTHE INFERENCE IS TO THE SIMPLER EXPLANATION: the named rival is co-firing from shared input with no synapse, and the discriminator is timescale
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content_tags.findings[11]1.0the tagger's words
content_tagsTHE STANDARD ASSUMPTION IS REJECTED BY NAME: under independence, broad-timescale effects alone can raise short-latency co-occurrence above chance
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content_tags.findings[12]1.0the tagger's words
content_tagsand an informal observation is given as further reason: the independence approach reliably yields ambiguous putative connections
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content_tags.findings[13]1.0the tagger's words
content_tagseach spike in each neuron was randomly and independently perturbed on a uniform interval of [-5,+5] ms, repeated independently 1,000 times to form the surrogate data sets
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content_tags.findings[14]1.0the tagger's words
content_tagsTHE NULL IS BUILT TO CONTAIN EVERYTHING BUT THE TIMESCALE OF INTEREST: jittering preserves broader-timescale rates, so what survives cannot be rate variation at tens of milliseconds
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content_tags.findings[15]1.0the tagger's words
content_tagsTHE CONFOUND IS DETECTABILITY: more spikes make a connection easier to detect, so a position-dependent rate could fake a position-dependent efficacy
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content_tags.findings[16]1.0the tagger's words
content_tagsSO DATA WERE DISCARDED TO REMOVE IT: spikes were deleted at random until rates were flat to within ten percent across maze segments, and the analysis rerun
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content_tags.findings[17]1.0the tagger's words
content_tagsthe argument being that with spikes uniform across positions, a remaining position dependence is unlikely to be a power effect
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content_tags.findings[18]1.0the tagger's words
content_tagsand the conclusion survived thinning in several cases
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content_tags.findings[19]1.0the tagger's words
content_tagsin some pairs thinning was unnecessary because rates were already flat while coincidences were confined to part of the maze
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content_tags.findings[20]1.0the tagger's words
content_tagsA PRIOR LITERATURE REATTRIBUTED: earlier work read such peaks as pyramidal-to-pyramidal, and physiological classification makes them mostly pyramidal-to-interneuron
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content_tags.findings[21]1.0the tagger's words
content_tagsneurons fired differentially by trajectory already in the central arm, where movement and head direction were indistinguishable
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content_tags.findings[22]1.0the tagger's words
content_tagsthe neuron already showed goal-specific elevated firing in the central arm, suggestive of goal representation
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content_tags.findings[23]1.0the tagger's words
content_tagsa lingering-odour confound was ruled out: stem-area differential firing showed no reliable relationship with nose-poke firing
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content_tags.findings[24]1.0the tagger's words
content_tagsand previous-trial reward position left only sparse traces in firing
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content_tags.findings[25]1.0the tagger's words
content_tagsenvironmental and motor differences alone cannot fully account for mPFC activity
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content_tags.findings[26]1.0the tagger's words
content_tagsindividual cells fired at specific locations while the population as a whole fired uniformly over the apparatus
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content_tags.findings[27]1.0the tagger's words
content_tagsone presynaptic partner's activity reduced its control of an interneuron while another's increased it, on the same cell
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content_tags.findings[28]1.0the tagger's words
content_tagswhich argues for synaptic mechanisms rather than passive membrane properties
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content_tags.findings[29]1.0the tagger's words
content_tagscoincident presynaptic discharge within 5 ms raised target firing probability supralinearly
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content_tags.findings[30]1.0the tagger's words
content_tagsTHE SILENT MAJORITY ACKNOWLEDGED: only about 10 to 25 percent of neurons in the sampled volume were active enough to cluster, and the estimates are said to need more direct methods
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content_tags.findings[31]1.0the tagger's words
content_tagsthe principal-to-interneuron ratio of 2.82 is lower than anatomy predicts, attributed to the method or to silent principal cells
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content_tags.findings[32]1.0the tagger's words
content_tags633 units were recorded from anterior cingulate and dorsal prelimbic cortex in four rats
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content_tags.findings[33]1.0the tagger's words
content_tagsrats performed at 92 percent correct at the time of recording
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content_tags.findings[34]1.0the tagger's words
content_tagsfunctional synaptic efficacy
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content_tags.definitions[0]the tagger's words
content_tagsspike train
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content_tags.definitions[1]the tagger's words
content_tagscoincident-time offset spike
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content_tags.definitions[2]the tagger's words
content_tagssurrogate data set
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content_tags.definitions[3]the tagger's words
content_tagsmonosynaptic
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also in: monosynaptic
content_tags.definitions[4]1.0the tagger's words
content_tagsjitter
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also in: jitter
content_tags.definitions[5]1.0the tagger's words
content_tagsthinning
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also in: thinning
content_tags.definitions[6]1.0the tagger's words
content_tagsposition in the maze
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content_tags.variables[1]the tagger's words
content_tagsjittered data sets
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content_tags.variables[5]the tagger's words
content_tagsfiring rates
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also in: firing rates
content_tags.variables[7]1.0the tagger's words
content_tagstrajectory
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also in: trajectory
content_tags.variables[8]1.0the tagger's words
content_tags633 — Total well-isolated single units recorded from medial prefrontal cortex across four rats
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content_tags.quantities[0]the tagger's words
content_tags495 (0.79%) of 62,408 — Cell pairs showing significant short-latency cross-correlogram peaks or troughs, i.e. putative monosynaptic connections
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content_tags.quantities[1]the tagger's words
content_tags2.82 ± 0.51 — Physiologically identified ratio of putative principal cells to inhibitory interneurons
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content_tags.quantities[2]the tagger's words
content_tags67 of 343 — Excitatory monosynaptic pairs showing statistically identifiable position dependence of their interaction
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content_tags.quantities[3]the tagger's words
content_tags12.7% and 10.7% — Proportions of neuron pairs showing firing-pattern-dependent depression and facilitation respectively
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content_tags.quantities[4]the tagger's words
content_tags92% correct — Mean behavioural accuracy of the rats on the odour-place matching task at the time of recording
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content_tags.quantities[5]the tagger's words
content_tags40% versus 22% — Fraction of trajectory-selective neurons in the side arms in layer 5 versus layer 2/3
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content_tags.quantities[6]the tagger's words
content_tags0.27 (62 cm) ± 0.17 (39 cm) — Mean standardized maze distance over which an individual neuron remained active (its firing 'lifetime')
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content_tags.quantities[7]the tagger's words
content_tags0.79% — share of directed cell pairs with a significant short-latency peak or trough
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content_tags.quantities[8]1.0the tagger's words
content_tags67 of 343 — excitatory pairs showing position-dependent monosynaptic interaction
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content_tags.quantities[9]1.0the tagger's words
content_tags1,200 μm — the greatest separation at which a connection was detected
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content_tags.quantities[10]1.0the tagger's words
content_tags2.82 ± 0.51 — observed principal-to-interneuron ratio, against about 4 from anatomy
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content_tags.quantities[11]1.0the tagger's words
content_tags92% correct — behavioural performance during recording
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content_tags.quantities[12]1.0the tagger's words

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