Large-scale recording of neurons by movable silicon probes in behaving rodents

Vandecasteele, M, Royer, Belluscio, Berényi, Diba, Fujisawa, Grosmark · Journal of Visualized Experiments: JoVE · 2012 · neuroscience · 10.3791/3568 · record Vandecasteele2012-large-scale-recording-of-neurons-by-movable
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Where this record's text came from. faceted 2026-08 by reading the locally held text (html); every evidence string verified as an exact substring of that text before merging. Claim relations left empty: the article relates itself to prior work only through numbered reference markers (e.g. '1-11', '17,19') and never names an author or a specific prior study in the body text, so no relation could be anchored to a verbatim sentence naming its target. method tagged system-or-tool-building because the protocol's substance is fabricating the microdrive/probe assembly; the only data shown are illustrative representative recordings (Figure 1), not a study. This is a JoVE video protocol, so much of the content is procedural instruction rather than argument. 2026-08-12 and 2026-08-15 schema-0.3 passes: every quote verified as an exact substring of the staged text and anchored at merge time; concept terms verified present; every edge endpoint an existing node.
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Tags on this paper

Every tag the record carries, each linking to every other paper in the corpus that carries it. A tag appears here whether or not its words occur in the article — the spans below are only the ones a sentence in this paper licenses. Of the 15 concept tags on this paper, none is grounded in a quoted sentence. All carry the tagger's gloss of what the term means in this paper, which is not something the article can license.
silicon probes microdrive local field potential unit activity neural assemblies cluster analysis theta impedance Faraday cage ground electrode shank correlational optogenetic cell types DiI Any break in the ground path (CSF to ground screw to copper wire to copper mesh to ground pin) leaves the headstage ungrounded, which is why movement artifacts and 50/60 Hz line noise appear in the recording A micro-drive protruding above a too-short Faraday cage picks up environmental electrical fields, acting as an antenna and injecting noise into the signal The copper-mesh cylinder encloses the headgear and shunts environmental fields and whisker-generated charge to ground, which is how it suppresses noise and slow-wave artifacts Placing the reference over the cerebellum exploits the fact that cerebellar LFP amplitude and midline muscle artifact are both minimal, so the referenced signal carries less contamination ideal microdrive electrophysiological signals cluster analysis of their waveforms system-or-tool-building

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second readingalternativesan independent re-reading of a facet, recorded whether or not it agrees
relation to other workclaim_relationshow this paper stands to another: agrees, contradicts, extends, qualifies
claim typeclaim_typethe shape of the claim: existence, mechanism, method, critique, prediction, null
concept edgeconcept_edgesan asserted relation between two concepts, with the sentence asserting it
content tagcontent_tagsa finding, definition, quantity, statistic or variable named in the paper
limitationlimitationswhat the paper says its evidence cannot support
mechanismmechanismshow the reported effect is said to come about
methodmethodhow the paper produced its evidence
object of studyobject_of_studywhat the paper is about — the thing it investigates
phenomenonphenomenonthe effect or regularity under study
sensesensesa distinct use of a term, quoted where the paper fixes it

The paper

The full text is not reproduced here. This article is not cleared for redistribution, so the page carries the tag layer only. Its evidence sentences are withheld; the grounding status remains with each tag, and the article itself is at https://doi.org/10.3791/3568. The reading view exists for the papers whose licence permits it.

Every tag on this page

alternatives 2 · claim_relations 2 · claim_type 1 · concept_edges 3 · content_tags 21 · limitations 8 · mechanisms 10 · method 1 · object_of_study 1 · phenomenon 1 · senses 4

Relations on this page carry a quotation for the claim. Where a relation was separately evidenced — and so tested against the vocabulary for its type — the row says so; none of 3 relations here carry that mark. The rest are the tagger’s reading of the sentence quoted beside them.

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facettagfieldconf.whose words
object_of_studyThe construction and implantation of a movable silicon-probe microdrive for chronic recording in freely moving rodents.
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object_of_studythe paper's words
claim_typemethod
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claim_typethe paper's words
phenomenonchronic large-scale silicon-probe recording in behaving rodents
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phenomenonthe paper's words
methodsystem-or-tool-building
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methodthe paper's words
mechanismsmechanisms
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mechanisms[0]the tagger's words
mechanismsmechanisms
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mechanisms[1]the tagger's words
mechanismsmechanisms
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mechanisms[2]the tagger's words
mechanismsmechanisms
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mechanisms[3]the tagger's words
mechanismsThe microdrive design is a set of trade-offs rather than an optimum, which is why different drives suit different applications
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mechanisms[4]the tagger's words
mechanismsThe probe shanks must be aligned parallel to the travel axis so that advancing moves them through tissue rather than cutting across it
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mechanisms[5]the tagger's words
mechanismsInsulation at the bonding area keeps cerebrospinal fluid from short-circuiting the connections
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mechanisms[6]the tagger's words
mechanismsGrounding is a continuous electrical path through fluid, screw, wire and mesh, and a break anywhere in it produces artefact or mains noise
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mechanisms[7]the tagger's words
mechanismsA protruding drive above the shielding acts as an antenna, which is fixed by grounding the drive itself
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mechanisms[8]the tagger's words
mechanismsRecording alone cannot settle cause and effect, so the remedy is to identify cell types and then drive them - which is where optogenetics enters
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mechanisms[9]the tagger's words
limitationsThe measurements are correlational, so causal relations among neuronal activity patterns cannot be resolved by this method.
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what this limits: any cause-effect reading of the recordings the method produces: GIVEN THE CORRELATIONAL NATURE OF THESE MEASUREMENTS, THE CAUSE-EFFECT RELATIONSHIP ... REMAINS INEVITABLY AMBIGUOUS
limitations[0]the tagger's words
limitationsOnly a single-probe implantation is demonstrated; multi-probe recording is asserted but not shown.
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what this limits: the multi-probe claim: WE ILLUSTRATED THE IMPLANTATION OF A SINGLE SILICON PROBE, and the rest CAN BE READILY ACCOMPLISHED AFTER SOME PRACTICE - asserted, not demonstrated
limitations[1]the tagger's words
limitationsThe filmed surgery is a demonstration in which several preparatory, surgical and postoperative steps are omitted.
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what this limits: the video as a protocol to work from: SEVERAL PREPARATORY STEPS, SURGICAL PRECAUTIONS AND POSTOPERATIVE PROCEDURES ARE NOT SHOWN/VISIBLE OR DISCUSSED
limitations[2]the tagger's words
limitationsThe authors state the correlational limit of the whole method: no amount of recording settles cause and effect
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what this limits: the same scope as limitations[0] - no quantity of recording settles cause and effect
limitations[3]the tagger's words
limitationsBoth the tissue and the probe are fragile, and the critical steps are the ones where either can be damaged
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what this limits: the success rate: CRITICAL STEPS ... ARISE FROM THE FRAGILITY OF BOTH BIOLOGICAL (BRAIN TISSUE) AND TECHNICAL (SILICON PROBE) MATERIALS
limitations[4]the tagger's words
limitationsInjury during preparation or afterwards destroys the unit activity the method exists to capture
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what this limits: the unit activity the method exists to capture: ANY INJURY TO THE BRAIN TISSUE ... WOULD RESULT IN DAMAGING THE CELLS AND JEOPARDIZING THE RECORDING OF UNIT ACTIVITY
limitations[5]the tagger's words
limitationsThe filmed procedure omits preparatory, surgical and postoperative steps for the sake of visibility
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what this limits: the same scope as limitations[2], stated outright: THE (MOCK) SURGERY SHOWN IN THIS VIDEO IS FOR DEMONSTRATION PURPOSES ONLY
limitations[6]the tagger's words
limitationsOnly a single-probe implantation is demonstrated, with multi-probe work left to practice
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what this limits: the same scope as limitations[1] - one probe shown, multiple probes asserted
limitations[7]the tagger's words
sensesrecording - as evidence about WHAT HAPPENS: which neurons fire when, which the method delivers at scale
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senses[0]1.0the tagger's words
sensesrecording - as evidence about WHAT CAUSES WHAT: which it cannot deliver at any scale, because the measurements are correlational
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senses[1]1.0the tagger's words
sensesthe limit - as a matter of SAMPLE SIZE: solvable by recording more neurons, perhaps every neuron in the volume
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senses[2]1.0the tagger's words
sensesthe limit - as a matter of KIND: not solvable by more recording at all, and requiring identification plus manipulation instead
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senses[3]1.0the tagger's words
alternativesclaim_type: method
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a second reading reaching the same value. Recorded: method — note: a second reading reaching the same value from a different sentence. Not applied - a person decides.
alternatives.claim_typethe tagger's words
alternativesphenomenon: chronic large-scale silicon-probe recording in behaving rodents
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a second reading reaching the same value. Recorded: chronic large-scale silicon-probe recording in behaving rodents — note: a second reading reaching the same value from a different sentence. Not applied - a person decides.
alternatives.phenomenonthe tagger's words
claim_relationsextends → the decade of ensemble recording work in small and large-brained animals including humans
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claim_relations[0]the tagger's words
claim_relationscritiques → the sufficiency of recording alone for understanding circuit mechanism
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claim_relations[1]the tagger's words
concept_edgesshank —part-of→ silicon probes
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strength of the relation: 1 Audit 2026-09-28: DOUBTFUL - read against its full span and kept, but the span speaks of 'a multiple shank silicon probe'; part-of is loose.
also in: shank · silicon probes
concept_edges[2]the tagger's words
concept_edgestheta —part-of→ local field potential
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strength of the relation: 1
concept_edges[4]the tagger's words
concept_edgescell types —part-of→ neural assemblies
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strength of the relation: 1 Audit 2026-09-28: DOUBTFUL - read against its full span and kept, but each cell type 'uniquely contributes to assembly behavior'; part-of is loose.
concept_edges[8]the tagger's words
content_tagscluster analysis
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content_tags.statistics[0]the tagger's words
content_tagsRecording from 64-128 sites simultaneously in a behaving rodent is now as easy as recording from 2 wire-electrode sites a decade earlier.
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content_tags.findings[0]the tagger's words
content_tagsSilicon-based multiple-site devices are effective for ensemble recording because they scale to many sites, occupy small volume, and have controlled geometric design.
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content_tags.findings[1]the tagger's words
content_tagsExample recordings show a prominent 8 Hz theta oscillation in the local field potential during exploration, with spiking superimposed on multiple shanks and sites.
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content_tags.findings[2]the tagger's words
content_tagsThe recorded electrophysiological signals depend both on which brain structure the probe sits in and on what the animal is doing at the time.
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content_tags.findings[3]the tagger's words
content_tagsEven large-scale silicon-probe recordings cannot establish cause and effect between neuronal activity patterns, because the measurements are purely correlational.
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content_tags.findings[4]the tagger's words
content_tagsThe authors project that refined large-scale probes combined with new mathematical and modelling work will allow recording from a representatively large fraction, or perhaps all, of the neurons in the volume a multi-shank probe surveys.
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content_tags.findings[5]the tagger's words
content_tagsrecording from 64 to 128 sites in a behaving rodent is now as easy as two sites were a decade earlier
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content_tags.findings[6]the tagger's words
content_tagsthe example recordings show theta-band field oscillation with spiking superimposed across shanks during exploration
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content_tags.findings[7]the tagger's words
content_tagsrecording from a representatively large fraction, or perhaps every neuron, in the surveyed volume is projected as reachable
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content_tags.findings[8]the tagger's words
content_tagsideal microdrive
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content_tags.definitions[0]the tagger's words
content_tagsmicrodrive
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also in: microdrive
content_tags.definitions[1]the tagger's words
content_tagselectrophysiological signals
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content_tags.variables[1]the tagger's words
content_tags20 000 Hz — wideband sampling rate of the chronic recordings, chosen so that both LFP oscillations and spikes can be studied
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content_tags.quantities[0]the tagger's words
content_tags35-70 micrometers — how far the probe is advanced per day (1/8 to 1/4 turn of the drive screw) while searching for the target structure
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content_tags.quantities[1]the tagger's words
content_tags64 to 128 — number of sites now routinely recorded simultaneously in a behaving rodent, versus 2 sites with wire electrodes a decade earlier
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content_tags.quantities[2]the tagger's words
content_tags8 Hz — frequency of the theta-band local field potential oscillation seen in CA1 during open-field exploration
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content_tags.quantities[3]the tagger's words
content_tags4-shank, 32-site — the probe configuration demonstrated
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content_tags.quantities[4]the tagger's words
content_tags20 000 Hz — the sampling rate allowing both field potentials and spikes from the same wideband signal
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content_tags.quantities[5]the tagger's words
content_tags64 to 128 sites — what is routine now against two sites a decade before
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content_tags.quantities[6]the tagger's words
content_tags8 Hz — the theta rhythm visible in the example hippocampal recording
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content_tags.quantities[7]the tagger's words

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