Discovery and quantification of plastic particle pollution in human blood

H.A. Leslie, Martin J. M. van Velzen, Sicco H. Brandsma, D. Vethaak, Juan J. García‐Vallejo, M.H. Lamoree · Environment International · 2022 · environmental-science · 10.1016/j.envint.2022.107199 · record leslie2022-discovery-and-quantification-of-pl
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Other fields define these differently the same word, defined another way in another discipline
microplastic 2 fields

Tags on this paper

Every tag the record carries, each linking to every other paper in the corpus that carries it. A tag appears here whether or not its words occur in the article — the spans below are only the ones a sentence in this paper licenses. Of the 11 concept tags on this paper, all 11 are grounded in a quoted sentence. All carry the tagger's gloss of what the term means in this paper, which is not something the article can license.
plastic particles microplastic nanoplastic pore size of 700 nm limits of quantification human health risk assessment protein corona Py-GC/MS procedural blank particulate matter field sampling blank observation
Method, as described: double-shot pyrolysis gas chromatography/mass spectrometry on whole blood from 22 healthy volunteers, targeting particles retained on a 700 nm filter

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relation to other workclaim_relationshow this paper stands to another: agrees, contradicts, extends, qualifies
claim typeclaim_typethe shape of the claim: existence, mechanism, method, critique, prediction, null
concept edgeconcept_edgesan asserted relation between two concepts, with the sentence asserting it
conceptconceptsan idea the paper works with, quoted where the paper uses it
content tagcontent_tagsa finding, definition, quantity, statistic or variable named in the paper
contributioncontributionwhat the paper claims to add
controlcontrolsan experiment run to eliminate a rival explanation
limitationlimitationswhat the paper says its evidence cannot support
mechanismmechanismshow the reported effect is said to come about
methodmethodhow the paper produced its evidence
object of studyobject_of_studywhat the paper is about — the thing it investigates
phenomenonphenomenonthe effect or regularity under study
research questionresearch_questionthe question the paper sets itself
sensesensesa distinct use of a term, quoted where the paper fixes it
transfertransfersa method or concept carried in from another field

The paper

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Every tag on this page

claim_relations 1 · claim_type 1 · concept_edges 1 · concepts 11 · content_tags 20 · contribution 1 · controls 7 · limitations 5 · mechanisms 5 · method 1 · object_of_study 1 · phenomenon 1 · research_question 1 · senses 5 · transfers 1

Relations on this page carry a quotation for the claim. Where a relation was separately evidenced — and so tested against the vocabulary for its type — the row says so; none of 1 relation here carry that mark. The rest are the tagger’s reading of the sentence quoted beside them.

facettagfieldconf.whose words
object_of_studyPLASTIC PARTICLES IN HUMAN BLOOD - a quantity nobody had measured, in a literature where the two available names for the particles do not have settled definitions
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object_of_studythe paper's words
research_questionwhether plastic particles cross into the human bloodstream at all, and whether a method sensitive enough to say so can be built
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research_questionthe paper's words
contributionthe first mass-concentration measurement of polymers in human blood - 1.6 ug/ml across 22 donors - and the double-shot Py-GC/MS method that produced it
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contributionthe paper's words
claim_typeexistence
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claim_typethe paper's words
phenomenonplastic particles absorbed across membranes and present in circulating blood, at a rate exceeding whatever removes them
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phenomenonthe paper's words
methodobservation
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also in: observation
methodthe paper's words
mechanismsTHE INFERENCE THE NUMBER LICENSES IS A RATE COMPARISON, NOT A QUANTITY: finding particles in blood `indicates that ... the rate of elimination via e.g. the biliary tract, kidney or transfer to and deposition in organs is slower than the rate of absorption into the blood`. A standing concentration is evidence about two rates and about neither one alone
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mechanisms[0]0.95the tagger's words
mechanismsand the paper is careful about what the blood compartment is: it `remains to be determined whether plastic particles are present in the plasma or are carried by specific cell types`. `In blood` is a location, and the mechanism of carriage is not in evidence
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mechanisms[1]0.95the tagger's words
mechanismsblood is the transport route: it irrigates the organs, so a particle that enters it can reach tissues, and its fate is renal or biliary elimination or deposition in liver, spleen or other organs
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mechanisms[2]0.95the tagger's words
mechanismscapillary diameter sets a physical ceiling on what can circulate, and anything at that size would itself affect microvascular flow
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mechanisms[3]0.95the tagger's words
mechanismsparticles may be localised in immune cells, stuck to proteins, lipids, each other or the vessel wall - so a mass concentration can be real while duplicates disagree
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mechanisms[4]0.9the tagger's words
limitationsTHE CONSTRUCT'S BOUNDS ARE TWO PHYSICAL OBJECTS AND THE PAPER SAYS SO BEFORE REPORTING A NUMBER: `Our operationally defined method targeted particles that could be retained on a filter with pore size of 700 nm ... The inner diameter of the needle used for venipuncture (0.514 mm) can be considered the upper size limit`. Everything below a filter pore and above a needle bore is outside the finding by construction
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limitations[0]0.95the tagger's words
limitationsAND THE INSTRUMENT DECIDES WHAT EXISTS: `Polypropylene was analysed but values were under the limits of quantification`. Absent from the blood and absent from the instrument are the same sentence here, and the study cannot separate them
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limitations[1]0.95the tagger's words
limitationsthe two available names for the object DO NOT HAVE SETTLED DEFINITIONS: `microplastic` has `no universally established definition` and is `often defined as ... up to 5 mm with no defined lower size limit`, while `nanoplastic` means <1 um in this literature and `<100 nm` IN THE NANOTECHNOLOGY FIELD. ONE WORD, TWO FIELDS, TWO THRESHOLDS
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limitations[2]0.95the tagger's words
limitationsthe sample is 22 donors and the paper calls it what it is - `a small set of donors` - in the same sentence as the headline concentration
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limitations[3]0.95the tagger's words
limitationsand the result is explicitly NOT a risk statement: `no absorption models can be validated and no statements about risk or no risk can be made` without measured internal exposure data. The measurement is a precondition for the question, not an answer to it
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limitations[4]0.95the tagger's words
transfersthe PM2.5/PM10 convention of reporting an operationally defined mass concentration per unit volume is adopted wholesale as the way to quantify plastic in blood
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transfers[0]0.95the tagger's words
controlsthe size window is fixed and declared before any measurement - a 700 nm filter pore below, a 0.514 mm needle bore above
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eliminates: any reading of the number as a total; it is the mass of what falls inside two physical limits, and the paper states both · outcome: held
controls[0]0.95the tagger's words
controlswhether polypropylene is absent from blood or below the assay's threshold
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eliminates: nothing, and the sentence cannot be made to: 'Polypropylene was analysed but values were under the limits of quantification' · outcome: failed
controls[1]0.95the tagger's words
controlswhether the measurement supports a risk claim
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eliminates: the risk reading, explicitly and in both directions: 'no statements about risk or no risk can be made' without measured internal exposure data - which this study provides for the first time and which is still only the precondition · outcome: held
controls[2]0.95the tagger's words
controlsdrawing blood through a closed all-glass vacutainer system with a steel needle and no plastic tubing or reservoir
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eliminates: plastic contamination introduced by the sampling equipment itself, which would manufacture the very finding being sought · outcome: held
controls[3]0.95the tagger's words
controlsidentifying the rubber vacutainer seal by ATR-FTIR to establish which polymer it is
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eliminates: the possibility that the one unavoidable non-glass component contributes to the targeted polymers · outcome: held
controls[4]0.95the tagger's words
controlsrunning the entire sampling system as a field blank before any sample
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eliminates: background contamination attributable to the collection apparatus rather than the donor · outcome: held
controls[5]0.9the tagger's words
controlsduplicate extraction and measurement of each sample where volume allowed, with multiple procedural blanks per series
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eliminates: single-measurement artefacts and uncorrected background drift between series · outcome: held
controls[6]0.95the tagger's words
conceptsplastic particles
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concepts[0]0.95the paper's words
conceptsmicroplastic
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also in: microplastic
concepts[1]0.95the paper's words
conceptsnanoplastic
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also in: nanoplastic
concepts[2]0.95the paper's words
conceptspore size of 700 nm
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concepts[3]0.95the paper's words
conceptslimits of quantification
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concepts[4]0.95the paper's words
conceptshuman health risk assessment
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concepts[5]0.95the paper's words
conceptsprotein corona
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concepts[6]0.95the paper's words
conceptsPy-GC/MS
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also in: Py-GC/MS
concepts[7]0.95the paper's words
conceptsprocedural blank
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concepts[8]0.95the paper's words
conceptsparticulate matter
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concepts[9]0.9the paper's words
conceptsfield sampling blank
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concepts[10]0.9the paper's words
senses`plastic particles` - A TERM THE AUTHORS COIN FOR THEIR OWN USE, because the field's two names will not hold still: `To circumvent the ambiguity of the terms microplastic and nanoplastic particles in this article we will refer to plastic particles and where appropriate define the size or size range`. The remedy for an unstable term is to stop using it and carry the number instead
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senses[0]0.95the tagger's words
senses`microplastic` - `no universally established definition exists`, and the common one - up to 5 mm - has NO LOWER BOUND AT ALL. A term whose extension is open at one end
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senses[1]0.95the tagger's words
senses`nanoplastic` - <1 um in this literature, `<100 nm` IN THE NANOTECHNOLOGY FIELD. ONE NAME, TWO FIELDS, A TENFOLD DIFFERENCE IN THE THRESHOLD, and the paper reports both rather than choosing
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senses[2]0.95the tagger's words
senses`a plastic particle in blood`, AS OPERATIONALISED HERE - anything retained on a 700 nm filter and small enough to pass a 0.514 mm needle. THE CONSTRUCT IS BOUNDED BY A FILTER PORE AND A NEEDLE BORE, in the authors' own words, and the phrase they use is `operationally defined`
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senses[3]0.95the tagger's words
senses`under the limits of quantification` - what the paper reports for polypropylene, and the one phrase in the record that cannot be read as a fact about blood. It is a fact about the assay
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senses[4]0.95the tagger's words
claim_relationsextends → Leslie and Depledge's and Vethaak and Legler's position that a human health risk assessment for plastic particles is not currently possible for want of exposure data
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claim_relations[0]0.95the tagger's words
concept_edgesplastic particles —operationalised-as→ pore size of 700 nm
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concept_edges[0]0.95the tagger's words
content_tagsTHE CONSTRUCT IS BOUNDED BY TWO PHYSICAL OBJECTS, IN THE AUTHORS' OWN WORDS: an `operationally defined` method targeting particles retained on a 700 nm FILTER PORE, with a 0.514 mm NEEDLE BORE as the upper limit
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content_tags.findings[0]0.95the tagger's words
content_tagsmicroplastic has no universally established definition and often no lower size limit; nanoplastic means under 1 micrometre here but under 100 nm in nanotechnology
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content_tags.findings[1]0.95the tagger's words
content_tagsand the authors' remedy is to ABANDON BOTH TERMS: `to circumvent the ambiguity ... we will refer to plastic particles and where appropriate define the size or size range`
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content_tags.findings[2]0.95the tagger's words
content_tags1.6 ug/ml mean sum quantifiable concentration across a SMALL SET OF DONORS - the first mass concentration of polymers measured in human blood
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content_tags.findings[3]0.95the tagger's words
content_tagspolypropylene was analysed, but its values were under the limits of quantification
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content_tags.findings[4]0.95the tagger's words
content_tagsat least some plastic particles can be bioavailable, and their elimination is slower than their absorption into the blood
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content_tags.findings[5]0.95the tagger's words
content_tagsand the result is explicitly not a risk statement in EITHER direction: `no statements about risk or no risk can be made` without measured internal exposure
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content_tags.findings[6]0.95the tagger's words
content_tagsit remains to be determined whether the particles are in the plasma or carried by specific cell types
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content_tags.findings[7]0.95the tagger's words
content_tagsmost available data covers particles above 10 or 50 micrometres
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content_tags.findings[8]0.95the tagger's words
content_tagsearlier FTIR analysis of human faeces showed that micro-sized plastic particles can be excreted via the gut
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content_tags.findings[9]0.9the tagger's words
content_tagsno one method fits all, so a combination of methods is required
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content_tags.findings[10]0.95the tagger's words
content_tagscoined to circumvent the ambiguity of `microplastic` and `nanoplastic`, and always carried with an explicit size or size range
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content_tags.definitions[0]0.95the tagger's words
content_tagsno universally established definition; often given as plastic particles up to 5 mm WITH NO DEFINED LOWER SIZE LIMIT
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also in: microplastic
content_tags.definitions[1]0.95the tagger's words
content_tagsnanometres - the filter pore that is the lower bound of the construct
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content_tags.quantities[0]0.95the tagger's words
content_tagsmillimetres - the venipuncture needle's inner diameter, and the upper bound
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content_tags.quantities[1]0.95the tagger's words
content_tagsmicrograms per millilitre, mean sum quantifiable concentration in 22 donors
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value source note: THE DESCRIPTION DRAWS ON THE PAPER BEYOND THIS QUOTE: the 22 donors are stated in the abstract ('in human whole blood from 22 healthy volunteers').
content_tags.quantities[2]0.95the tagger's words
content_tagsthe proportion of body weight made up by blood
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content_tags.quantities[3]0.95the tagger's words
content_tagscapillary diameter, which bounds the particle size that can circulate
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content_tags.quantities[4]0.95the tagger's words
content_tagsthe number of volunteers and the collection protocol
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content_tags.quantities[5]0.95the tagger's words
content_tagslimits of quantification
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content_tags.statistics[0]0.95the tagger's words

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